Unprecedented enhancement and preservation of the peroxidase activity of cytochrome- packaged with ionic liquid-modified gold nanoparticles by offsetting temperature and time stresses.

Inspired by the biocompatibility of ionic liquids and their dexterousness for the preservation of enzyme structure and activity, herein, the interactions of Cyt- with naked AuNPs and four IL-mediated AuNPs, which were formed by the fabrication of ILs with common cation 1-ethyl-3-methyl-imidazolium (EMIM) and different anions, to obtain AuNP-IL1 [(BF4) anion], AuNP-IL2 [(CHOSO) anion], AuNP-IL3 [(CHCHOSO) anion], and (AuNP-IL4) [Cl anion], were studied. Through this work, the peroxidase activity observed in the presence of a lower concentration IL-AuNPs is exceptionally increased (16 fold). IL-AuNPs preferentially counteract the temperature gradient change and long-term solvent preservation effects while persistently maintaining the Cyt- peroxidase activity without much depreciation.

Protein packaging in ionic liquid mixtures: an ecofriendly approach towards the improved stability of β-lactoglobulin in cholinium-based mixed ionic liquids.

Ionic liquid (IL)-protein interaction studies are well documented; nonetheless, some families of ILs possess high toxicity which leads to the perturbation of biomolecules. However, ILs can be suitably designed by tweaking the constituent ions and also by amalgamating the mixture that stabilises the protein against the denaturing effect of another IL. To address this, the present study has explored the role of IL mixtures in imparting structural stability to protein β-lactoglobulin (β-LG).

A biophysical strategy to examine the impact of newly synthesized polymerizable ammonium-based ionic liquids on the structural stability and proteolytic activity of stem bromelain.

The present manuscript reports the synthesis and characterization of polymerizable ammonium-based ionic liquids (ILs) and explores their influence on structural stability of stem bromelain (BMN) by using various biophysical techniques. Thermal fluorescence results showed that N-ethyl-2-(methylacryloyloxy)-N,N-dimethylethan-1-ammonium bromide (IL) (at lower concentration (0.1 mg/mL)) is found to be increasing the thermal stability of BMN which can be evident from the transition temperature (T) for BMN in IL (68.

Insight into impact of choline-based ionic liquids on bovine β-lactoglobulin structural analysis: Unexpected high thermal stability of protein.

In this present work, we report the effects of different concentrations of various cholinium-based ionic liquids (ILs) on the structural and thermal stability of β-lactoglobulin (β-LG). Results indicated that anions in ILs have played an important role in affecting the thermal and structural stability of β-LG. Biomolecular interactions between β-LG and ILs are carried out by differential scanning calorimetry (DSC), different spectroscopic and dynamic light scattering (DLS).