High sensitive PCR method for detection of pathogenic Leptospira spp. in paraffin-embedded tissues.

This study describes the development and application of a new PCR assay for the specific detection of pathogenic leptospires and its comparison with a previously reported PCR protocol. New primers were designed for PCR optimization and evaluation in artificially-infected paraffin-embedded tissues. PCR was then applied to post-mortem, paraffin-embedded samples, followed by amplicon sequencing.

Molecular epidemiology of human papillomavirus infections in cervical samples from cuban women older than 30 years.

Objective: This study aimed to provide information about the molecular epidemiology of human papillomavirus (HPV) in a group of Cuban women.

Materials And Methods: DNA from cervical samples was analyzed using a quantitative real-time polymerase chain reaction (PCR), which detects 6 of the clinically most relevant high-risk HPV types. Furthermore, end point PCR and sequencing were performed.

Low genetic diversity of Pneumocystis jirovecii among Cuban population based on two-locus mitochondrial typing.

Genotypes of two different loci of the Pneumocystis jirovecii mitochondrial gene were studied in specimens from a total of 75 Pneumocystis pneumonia patients in Spain, France and Cuba. A new genotype of the mitochondrial small subunit rRNA gene of P. jirovecii (160A/196T) was identified, which was revealed to be the most common in these three countries, especially in Cuba where its proportion reached 93.