Three-Dimensional Printing/Bioprinting and Cellular Therapies for Regenerative Medicine: Current Advances.

The application of three-dimensional (3D) printing/bioprinting technologies and cell therapies has garnered significant attention due to their potential in the field of regenerative medicine. This paper aims to provide a comprehensive overview of 3D printing/bioprinting technology and cell therapies, highlighting their results in diverse medical applications, while also discussing the capabilities and limitations of their combined use. The synergistic combination of 3D printing and cellular therapies has been recognised as a promising and innovative approach, and it is expected that these technologies will progressively assume a crucial role in the treatment of various diseases and conditions in the foreseeable future.

De novo and inherited dominant variants in U4 and U6 snRNAs cause retinitis pigmentosa.

The U4 small nuclear RNA (snRNA) forms a duplex with the U6 snRNA and, together with U5 and ∼30 proteins, is part of the U4/U6.U5 tri-snRNP complex, located at the core of the major spliceosome. Recently, recurrent variants in the U4 RNA, transcribed from the gene, and in at least two other genes were discovered to cause neurodevelopmental disorder.

Adolescents and parents experiencing parental cancer: construction of a nursing intervention model.

Objective: To develop a nursing intervention model for adolescents and parents experiencing parental cancer.

Method: Multimethod research, which integrated five studies: a scoping review and four qualitative studies. Considering the results of these studies, a nursing intervention model was constructed based on two theoretical frameworks, such as the A Model of Children's Adjustment to Parental Cancer, for adolescents, and Neuman Systems Model, for parents, and on a semantic framework, such as Nursing Ontology.

Stem Cell-Based Therapies for Glaucoma Treatment: A Review Bridging the Gap in Veterinary Patients.

Retinal diseases are characterized by progressive damage to retinal cells, leading to irreversible vision loss. Among these, glaucoma stands out as a multifactorial neurodegenerative disease involving elevated intraocular pressure, retinal ganglion cell apoptosis, and optic nerve damage, ultimately resulting in blindness in both humans and dogs. Stem cell-based therapies have emerged as a promising therapeutic option for such conditions due to their regenerative and neuroprotective potential.

Mechanistic Insights into Succinic Acid as an Adjuvant for Ciprofloxacin in Treating Growing Within Cystic Fibrosis Airway Mucus.

is a major cause of chronic respiratory infections in patients with cystic fibrosis (CF), with biofilm formation contributing to its persistence and antibiotic resistance. This study aimed to gain insights into the mechanistic action of succinic acid as a ciprofloxacin adjuvant against clinically relevant CF isolates, including small colony variants and mucoid strains, and a ciprofloxacin-resistant strain grown within CF dense mucus. Time-kill assays in artificial CF mucus, along with planktonic and surface-attached biofilm experiments, were used to assess the activity of succinic acid alone and in combination with sublethal ciprofloxacin concentrations.

New Strains of the Entomopathogenic Nematodes , , and for White Grub Management.

White grubs possess natural defense mechanisms against entomopathogenic nematodes (EPNs). Hence, EPN isolates that naturally infect white grubs tend to be among the most effective biological control agents of white grubs. We tested the virulence of four EPN isolates recently isolated from infected white grubs in turfgrass areas in central New Jersey, USA against third-instar larvae of , , and , which are pests of turfgrass and ornamental plants in the northeastern USA.

Isolation, Expansion, and Characterization of Rat Hair Follicle Stem Cells and Their Secretome: Insights into Wound Healing Potential.

Stem cells are capable of self-renewal and differentiation into various specialized cells, making them a potential therapeutic option in regenerative medicine. This study establishes a comprehensive methodology for isolating, culturing, and characterizing rat hair follicle stem cells. Hair follicles were harvested from Sprague-Dawley rats and subjected to two different isolation techniques.

Dietary nitrate supplementation very slightly mitigates the oxidative stress induced by high-intensity training performed in normobaric hypoxia.

Oxidative stress is augmented under hypoxic environments, which may be attenuated with antioxidant supplementation. We investigated the effects of dietary nitrate (NO-) supplementation combined with high-intensity training performed under hypoxic conditions on antioxidant/pro-oxidant balance. Thirty trained participants were assigned to one of three groups - HNO: hypoxia (13% FO) + NO-; HPL: hypoxia + placebo; CON: normoxia (20.

Equine endometrial bacteria inhibition by metabolite and extracellular vesicles of Lactobacillus acidophilus and lactiplantibacillus plantarum.

Endometritis is one of the main reproductive disorders in mares and due to the increasing prevalence of antibiotic resistance, the use of probiotics in the prevention and treatment of endometritis in mares has gained interest, given their potential to restore and maintain a healthy uterine microbiota. Therefore, the aim of this study was to evaluate the antimicrobial properties of total metabolites of Lactobacillus acidophilus (LA) and Lactiplantibacillus plantarum (LP) against common equine endometrial pathogenic bacteria in vitro (Acinetobacter baumannii, Escherichia coli (1), Escherichia coli (2), Escherichia coli (3), Escherichia coli (4), Enterobacter cloacae, Streptococcus equi, Staphylococcus warneri, Actinobacillus equi and Klebesiella pneumoniae), as well as to assess their low molecular weight metabolites (loM) and extracellular vesicle (EVs) inhibition capacity over a multidrug-resistant E. coli isolated from mares with clinical endometritis.

Genetic modulation of RNA splicing rescues BRCA2 function in mutant cells.

Variants in the hereditary cancer-associated and genes can alter RNA splicing, producing transcripts that encode internally truncated yet potentially functional proteins. However, few studies have quantitatively analyzed variant-specific splicing isoforms. Here, we investigated cells heterozygous and homozygous for the :c.

Evaluation of the interface of metallic-coated biodegradable polymeric stents with prokaryotic and eukaryotic cells.

Polymeric coronary stents, like the ABSORB™, are commonly used to treat atherosclerosis due to their bioresorbable and cell-compatible polymer structure. However, they face challenges such as high strut thickness, high elastic recoil, and lack of radiopacity. This study aims to address these limitations by modifying degradable stents produced by additive manufacturing with poly(lactic acid) (PLA) and poly(ε-caprolactone) (PCL) with degradable metallic coatings, specifically zinc (Zn) and magnesium (Mg), deposited via radiofrequency (rf) magnetron sputtering.

Innovative Ink-Based 3D Hydrogel Bioprinted Formulations for Tissue Engineering Applications.

Three-dimensional (3D) models with improved biomimicry are essential to reduce animal experimentation and drive innovation in tissue engineering. In this study, we investigate the use of alginate-based materials as polymeric inks for 3D bioprinting of osteogenic models using human bone marrow stem/stromal cells (hBMSCs). A composite bioink incorporating alginate, nano-hydroxyapatite (nHA), type I collagen (Col) and hBMSCs was developed and for extrusion-based printing.

Chronic rhinosinusitis with nasal polyps (CRSwNP) symptom verbal response scales: content validity testing for use in adults with CRSwNP.

Background: PRO measures of symptoms in clinical trials have historically utilized visual and numerical scales but verbal descriptors may make it easier for patients to clearly differentiate between response options. This study assessed content validity and meaningful change in five verbal response scales (VRSs) used to assess chronic rhinosinusitis with nasal polyp (CRSwNP) symptom severity.

Methodology: This qualitative, semi-structured interview study recruited adults from the US, Germany, and China with confirmed moderate-to-severe CRSwNP.

Nasal telangiectatic osteosarcoma with direct extension to the brain in a domestic shorthair cat.

Case Summary: This case report describes the clinical and pathological features of telangiectatic osteosarcoma (TOS) with brain invasion in a 4-year-old female domestic shorthair cat. The cat presented with respiratory distress, epistaxis, anorexia and significant nasal obstruction. A rhinoscopy revealed an amorphous white neoformation in the left nasal cavity.

The finger-ring test as an indicator of muscle mass estimated by dual-energy X-ray absorptiometry in an adult community-dwelling population.

Introduction: The finger-ring test (FRT) offers a simple method to screen older adults at increased risk of sarcopenia.

Objective: To investigate the association between the FRT and lean soft tissue mass estimated by dual-energy X-ray absorptiometry (DXA), and to further elucidate the FRT as an indicator of muscle mass, while accounting for potential confounders, in a community-dwelling population.

Materials And Methods: Within this cross-sectional study 430 community-dwelling individuals aged 18 to 79 (286 women (66.

Competing at Altitude Reduces In-Match Physical Demands of Professional Soccer Players Compared With Sea Level.

Purpose: This study examined whether physical demands during soccer matches differ between sea level and altitude, considering variations by playing position.

Methods: Thirty-seven professional players were monitored during 22 matches (11 at sea level and 11 at altitudes of 2200-4090 m) with global navigation satellite systems. Independent mean differences were used to compare in-match physical demands (ie, total distance, distance covered at specific speed intervals, accelerations [ACCs] and decelerations [DECs], and maximal speed) between locations for 5 playing positions (central defenders, fullbacks, central midfielders, wide midfielders, and forwards).

Osteoprotegerin and Vascular Dysfunction in Patients with Stage 3 Chronic Kidney Disease and Those without Renal Dysfunction: A Case-Control Study.

Background: Osteoprotegerin (OPG) is a marker of vascular calcification and cardiovascular risk in patients with chronic kidney disease (CKD).

Objective: This study aimed to compare and correlate OPG values with flow-mediated dilation (FMD) and pulse wave velocity (PWV) measurements in patients in stage 3 CKD and those without renal dysfunction.

Methods: This case-control study was conducted in a specialized hypertension center in 2022.

GPATCH11 variants cause mis-splicing and early-onset retinal dystrophy with neurological impairment.

Here we conduct a study involving 12 individuals with retinal dystrophy, neurological impairment, and skeletal abnormalities, with special focus on GPATCH11, a lesser-known G-patch domain-containing protein, regulator of RNA metabolism. To elucidate its role, we study fibroblasts from unaffected individuals and patients carrying the recurring c.328+1 G > T mutation, which specifically removes the main part of the G-patch domain while preserving the other domains.

Synchronous Pleural and Peritoneal Mesothelioma in a Free-Ranging Capuchin Monkey (Sapajus libidinosus).

A free-ranging capuchin monkey developed a synchronous biphasic pleural and peritoneal mesothelioma, a rare form of this tumor. Exophytic nodules were dispersed over the serosal surfaces, showing marked microscopical malignant features composed of both epithelioid and spindeloid neoplastic cells immunopositive for Pan-Cytokeratin, Cytokeratin 5/6, Vimentin, WT-1, and D2-40.

The immune landscape of murine skeletal muscle regeneration and aging.

Age-related alterations in the immune system are starting to emerge as key contributors to impairments found in aged organs. A decline in regenerative capacity is a hallmark of tissue aging; however, the contribution of immune aging to regenerative failure is just starting to be explored. Here, we apply a strategy combining single-cell RNA sequencing with flow cytometry, histological analysis, and functional assays to perform a complete analysis of the immune environment of the aged regenerating skeletal muscle on a time course following injury with single-cell resolution.