Urinary volatile fingerprint based on mass spectrometry for the discrimination of patients with lung cancer and controls.

Profile signals of urine samples corresponding to patients with lung cancer and controls were obtained using a non-separative methodology. The method is based on the coupling of a headspace sampler, a programed temperature vaporizer and a mass spectrometer (HS-PTV-MS). With only a centrifugation step as prior sample treatment, the samples were subjected to the headspace generation process and the volatiles generated were introduced into the PTV where they were trapped in the Tenax® packed liner while the solvent was purged.

Headspace-programmed temperature vaporizer-mass spectrometry and pattern recognition techniques for the analysis of volatiles in saliva samples.

A rapid method for the analysis of volatiles in saliva samples is proposed. The method is based on direct coupling of three components: a headspace sampler (HS), a programmable temperature vaporizer (PTV) and a quadrupole mass spectrometer (qMS). Several applications in the biomedical field have been proposed with electronic noses based on different sensors.

Headspace-programmed temperature vaporization-mass spectrometry for the rapid determination of possible volatile biomarkers of lung cancer in urine.

We propose a new method for the rapid determination of five volatile compounds described in the literature as possible biomarkers of lung cancer in urine samples. The method is based on the coupling of a headspace sampler, a programmed temperature vaporizer in solvent-vent injection mode, and a mass spectrometer (HS-PTV-MS). This configuration is known as an electronic nose based on mass spectrometry.

Headspace generation coupled to gas chromatography-mass spectrometry for the automated determination and quantification of endogenous compounds in urine. Aldehydes as possible markers of oxidative stress.

A methodology for the determination of five aldehydes in urine has been developed based on the coupling of a headspace generation sampler with a gas chromatography-mass spectrometry system equipped with a programmed temperature vaporizer. This instrumental configuration minimizes sample manipulation and allows the determination of these compounds without the need for a derivatization step so that the methodology is largely shortened and simplified. An experimental design was carried out in order to optimize the headspace sampling.

Coupling of microextraction by packed sorbents with gas chromatography with ionic liquid stationary phases for the determination of haloanisoles in wines.

A new method based on the coupling of microextraction by packed sorbents (MEPS) and gas chromatography with ionic liquid stationary phases for the determination of four haloanisoles in wine matrices is proposed. The analytes were concentrated in a C18 barrel insert and needle (BIN), desorbed with a small volume of an organic solvent, and determined by gas chromatography with electron-capture detection (GC-μECD). The influence of several factors on the efficiency of extraction, washing and elution steps was investigated.