Exploring the exocellular fungal biopolymer botryosphaeran for laccase-biosensor architecture and application to determine dopamine and spironolactone.

Laccase was immobilized on a glassy carbon electrode layered with multi-walled carbon nanotubes using a film of botryosphaeran, a fungal (1 → 3)(1 → 6)-β-D-glucan. This novel biosensing platform was characterized by electrochemical impedance spectroscopy and scanning electron microscopy, and applied for the determination of dopamine. Experimental variables such as enzyme concentration, pH value and operational parameters of the electroanalytical technique were optimized.

Exploiting the high oxidation potential of carisoprodol on a boron-doped diamond electrode: an improved method for its simultaneous determination with acetaminophen and caffeine.

A boron-doped diamond electrode (BDDE) was employed for the first time in the development of a voltammetric method for the simultaneous determination of acetaminophen (ACT), caffeine (CAF) and carisoprodol (CAR). CAR presents a high oxidation potential (2.0 V) and this electrode is suitable for this purpose due to its wide electrochemical potential window.