[Ige reactivity of a recombinant multi-epitope protein designed from allergens of interest in the tropics - preliminary findings].

Objective: The objective of the present study was to design a multi-epitope protein from and APD allergens and to evaluate its IgE reactivity preliminarily.

Methods: Using computational tools, a molecule containing multiple "T" epitopes of allergens derived from and APD was designed "" This multi-epitope protein (MP1) was expressed using an system and purified by affinity chromatography using Ni-NTA agarose. Anti-MP1 and anti-HDM extract IgE reactivity was evaluated by Dot-Blot and indirect ELISA from sera of HDM-allergic patients and non-allergic individuals from Barranquilla-Colombia.

[Novel tropomyosin consensus B and T epitopes involved in cross-reactivity between 10 different species. An in silico study].

Objective: This study aimed to identify by in silico methods tropomyosin consensus B and T epitopes of shrimp species, house dust mites, insects, and nematodes associated with allergic diseases in tropical countries.

Methods: In silico analysis included tropomyosin from mites (Der p 10, Der f 10, Blo t 10), insects (Aed a 10, Per a 7, Bla g 7), shrimp (Lit v 1, Pen m 1, Pen a 1), and nematode (Asc l 3) all sequences were taken from the UniProt database. Linear IgE epitopes were predicted with AlgPred 2.

[IgE reactivity of sera from patients with autoimmune diseases against a multi-epitope protein constructed from T epitopes of Ascaris lumbricoides allergens].

Objective: To evaluate the IgE reactivity of sera in patients suffering from type 1 diabetes (T1D), lupus nephritis (LN) and juvenile idiopathic arthritis (JIA) against a molecule constructed from T epitopes of allergens.

Methods: We designed and expressed a synthetic multi-epítope protein named MP1 from A. lumbricoides and house dust mites allergens.