Publications by authors named "Ana Molineri"

Article Synopsis
  • A large-scale survey conducted in Latin America found that on average, 30.4% of managed honey bee colonies and 39.6% of stingless bee colonies were lost each year over a two-year period (2016-2018).
  • Summer losses for stingless bees (30.9%) were higher compared to winter losses (22.2%), while honey bee losses did not show the same seasonal pattern.
  • The survey revealed significant differences in colony loss rates between countries and over the years, indicating challenges in maintaining bee colony health and economic viability for those involved in beekeeping.
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The aim of this study was to compare the effects of feeding pasteurized waste milk or saleable milk to calves on weight, health and emergence of antimicrobial resistance in strains isolated from those calves. An experimental study under field conditions on a commercial pasture-based Argentinian dairy farm was carried out. Forty Holstein calves were assigned randomly to either pasteurized waste milk (PWM) or non-pasteurized saleable milk (SM).

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Bovine genital campylobacteriosis (BGC) and bovine trichomonosis (BT) are sexually transmitted diseases (STDs) that affect bovine breeding herds, decreasing their reproductive efficiency. The objective of this work was to estimate the prevalence of these diseases and their temporal-spatial distribution in the province of Formosa, Argentina. The cross-sectional study conducted between 2018 and 2021 included a total of 15,571 bulls, inter-herd prevalence being 29.

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Article Synopsis
  • The study focused on the presence of antibodies against Neospora caninum, Toxoplasma gondii, and Brucella melitensis in sheep and goats in central Argentina, which can lead to abortion and include zoonotic pathogens.
  • Out of 4,783 goats and 1,524 sheep tested, the seroprevalence for T. gondii was 41.2% in sheep and 29.7% in goats, while N. caninum had 17.2% in sheep and 14% in goats, with no positive cases for B. melitensis.
  • Significant spatial clustering for T. gondii positivity was found, particularly along the Parana River, with animals from certain provinces at
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The COVID-19 pandemic remained worldwide for almost three years, but little is known about the dynamics of humoral immune response to the third dose over time and its protection from infection. Our aim was to assess the humoral immune response after the third dose of the different vaccines administered to SARS-CoV-2 naive and previously infected individuals, and its correlation with protection in an academic community. For each person studied (185), three blood samples were taken between December 2021 and July 2022, one month apart.

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Streptococcus uberis is one of the most common pathogens associated with bovine mastitis, commonly treated with antimicrobials (AM), favoring the appearance of antimicrobial resistance (AMR). The objective of this work was to determine the proportion of phenotypic AMR among S. uberis isolated worldwide from bovine intramammary infections between the years 1983-2022, and to assess the variables associated by means of a systematic review and metanalysis.

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  • Bovine leukemia virus (BLV) is prevalent in Argentine dairy cattle, but this study assesses its presence in beef cattle across thirteen provinces in Argentina, involving 5827 serum samples from 76 farms.
  • The study found a herd-level seroprevalence rate of 71.05%, with individual animal-level seroprevalence averaging 7.23%, indicating significant BLV infection rates in beef cattle, especially in most provinces.
  • Key management practices linked to higher BLV prevalence included using farm-raised animals for replacements, natural breeding methods, and late calf weaning, highlighting the need for awareness given Argentina's major role in global beef production.
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  • Infestation with Varroa destructor parasites negatively impacts the survival of honey bee colonies (Apis mellifera), particularly in winter, making it crucial to assess infestation levels and apply treatments in autumn.
  • The study highlights that re-invasion of treated colonies by Varroa mites can occur due to various factors, such as the status of untreated colonies and the layout of colonies within the apiary.
  • Research conducted across six apiaries revealed that colonies arranged in a linear pattern and with more available brood are more susceptible to Varroa re-invasion, emphasizing the importance of strategic colony management for effective pest control.
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Bovine pestiviruses are the causative agents of bovine viral diarrhea, a disease that causes severe economic losses in cattle. The aim of this study was to improve their diagnosis by developing a RT-qPCR to detect bovine pestiviruses A, B and H; and to set up a protocol for collecting, shipping and preserving bovine pestiviral RNA on filter papers. The developed RT-qPCR showed high sensitivity in detecting these viruses in different matrices: viral stocks, semen and serum samples.

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Varroa destructor is one of the most important sanitary threats for the beekeeping industry and so far disease control is based mainly on chemical treatment. However, a long-term solution may arise from studying natural surviving colonies of Apis mellifera. We compared the Varroa infestation rate in six commercial colonies that received annual treatment against mites and six non-treated colonies that survived in absence of any treatment for the last 6 years.

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Our objective was to evaluate the efficacy of intramammary administration, at drying-off, of a Panax ginseng extract (PGe) combined with cephalexin (Ceph) on the post-calving bacteriological cure rate of pre-existing intramammary infections (IMI) and on the occurrence of new IMI during the dry period. In addition, milk yield and somatic cell count (SCC) in the post-treatment lactation were evaluated. One hundred and eight late-lactation cows were randomly divided into two experimental groups and were treated at drying-off with Ceph alone or PGe combined with Ceph.

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Staphylococcus aureus, one of the main contagious mastitis pathogens worldwide, is characterized for causing chronic intramammary infections that respond poorly to antimicrobial therapy, disseminating within the herd leading to high economic losses. The aim of this study was to determine the prevalence of phenotypic resistance to antimicrobial agents among S. aureus collected worldwide in the context of bovine intramammary infections between the years 1969-2020.

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The aims of the research reported here were to identify potential risk factors associated with the presence of Staphylococcus aureus intramammary infection (IMI) in pre partum dairy heifers on 17 dairy farms from three provinces of Argentina and to characterize, at molecular level, isolates from those heifers and lactating cows from two selected herds. A total of 1474 heifers and 4878 lactating cows were studied. The prevalence of Staphylococcus aureus IMI in the heifers, heifers at quarter level and lactating cow mammary quarters was 14.

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The presence of Varroa destructor in colonies of Apis mellifera is explained by the interaction among a number of factors including beekeeping practices and surrounding environment features. The aim of this study was to evaluate the relative impact of environment geographical region and beekeeping management on Varroa infestation levels throughout a year. A monitoring study was carried out during 2015 in north-central regions from Argentina, consisting of three sampling dates: 1) autumn survey before autumn acaricide treatment; 2) autumn survey after autumn acaricide treatment and 3) spring survey.

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Beekeepers all across the world are suffering important losses of their colonies, and the parasitic mites Varroa destructor and Nosema sp, as well as several bee viruses, are being pointed out as the possible causes of these losses, generally associated with environmental and management factors. The objective of the present study was to evaluate the presence of seven virus species (Deformed wing virus -DWV-, Acute bee paralysis virus -ABPV-, Chronic bee paralysis virus -CBPV-, Black queen cell virus -BQCV-, Kashmir bee virus -KBV-, Israeli acute bee paralysis virus -IAPV-, and Sacbrood bee virus -SBV), as well as the prevalence of Nosema sp. and Varroa destructor, and their possible associated factors, under temperate and subtropical climate conditions in Argentinean colonies.

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In Argentina, bee virus studies are still incipient, and there are no studies regarding the climatic effect. The aim of this study was to assess and compare the presence of honeybee viruses in different climatic regions from Argentina. A total of 385 colonies distributed in five Argentinean eco-regions were examined to evaluate the percentage of infestation with Varroa destructor and the presence of seven virus species (Deformed wing virus, DWV; Acute bee paralysis virus, ABPV; Chronic bee paralysis virus, CBPV; Black queen cell virus, BQCV; Kashmer bee virus, KBV; Israeli acute bee paralysis virus, IAPV; and Sacbrood bee virus, SBV) after honey yield.

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Varroa destructor is one of the major threats to honey bee colonies. The mite abundance in the colonies is affected by environmental conditions as well as by beekeeping management. The aim of this study was to recognize the main drivers associated with autumn V.

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A total of 361 colonies from 59 apiaries located in two temperate and three subtropical eco-regions were examined during the post-harvest period to determine distribution and prevalence of Nosema spp. Apiaries from subtropical eco-regions showed a lower spore count than those from temperate eco-regions. Pure N.

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Varroa destructor is considered one of the main threats to worldwide apiculture causing a variety of physiological effects at individual and colony level. Also, Varroa mites are often associated with several honey bee viruses presence. Relatively low levels of Varroa during the spring, at the beginning of the honey yield season, can have a significant economic impact on honey production and colony health.

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Information on the long‑term consequences of Nosema ceranae to honey bee lifespan and effectiveness of Nosema control with fumagillin is scarce and not always consistent. Our objective in this study was to evaluate the effectiveness of the antibiotic fumagillin to control N. ceranae in hives in East‑Central Argentina.

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Honey bee colonies are threatened by multiple factors including complex interactions between environmental and diseases such as parasitic mites and viruses. We compared the presence of honeybee-pathogenic viruses and Varroa infestation rate in four apiaries: commercial colonies that received treatment against Varroa and non-treated colonies that did not received any treatment for the last 4 years located in temperate and subtropical climate. In addition, we evaluated the effect of climate and Varroa treatment on deformed wing virus (DWV) amounts.

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Objectives: a) To identify possible risk factors associated with rural accidents in women, b) to describe women's knowledge about zoonosis, c) to describe women's risk perception about farming, d) to describe mothers' risk perception about children's activities in rural settings, e) to estimate the initial age of exposure to the rural setting and its dangers, and f) to identify possible risk factors associated with rural accidents in children.

Materials And Methods: Cross-sectional design. Women with children that live in the countryside were surveyed (n=24).

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The aim of this study was to evaluate the level of awareness of zoonoses among rural workers and their potential associations with socio-demographic factors. A cross-sectional study was performed by holding personal interviews (N=110, n=94) using a structured questionnaire. The statistical analysis included the χ(2) test, the Student's t test and Pearson and Spearman correlations.

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Synopsis of recent research by authors named "Ana Molineri"

  • - Ana Molineri's recent research focuses primarily on the health and sustainability of agricultural systems in Argentina, with significant investigations into honey bee colony losses, bovine diseases, and antimicrobial resistance in livestock.
  • - A notable finding from her work is the alarming rate of managed honey bee and stingless bee colony losses in Latin America, highlighting the urgent need for effective management strategies and further research in apiculture.
  • - Additionally, her studies encompass the prevalence of sexually transmitted diseases in bovine populations and the impacts of feeding practices on antimicrobial resistance, emphasizing the interplay between animal management practices and public health concerns.