Induction of pigment production through media composition, abiotic and biotic factors in two filamentous fungi.

In addition to plant-derived, fungal pigments have become an alternative in respect to synthetic ones. Besides sp., several pigment-producing fungi do not have culture conditions well-established yet.

ASG2 is a farnesylated DWD protein that acts as ABA negative regulator in Arabidopsis.

The tagging-via-substrate approach designed for the capture of mammal prenylated proteins was adapted to Arabidopsis cell culture. In this way, proteins are in vivo tagged with an azide-modified farnesyl moiety and captured thanks to biotin alkyne Click-iT® chemistry with further streptavidin-affinity chromatography. Mass spectrometry analyses identified four small GTPases and ASG2 (ALTERED SEED GERMINATION 2), a protein previously associated to the seed germination gene network.