Characterization of the plasmidic or chromosomal cpe gene and metabolic activities in Clostridium perfringens isolates from food in San Luis--Argentina.

Food poisoning and non-food poisoning illnesses due to C. perfringens (by enterotoxin production) have been associated to chromosomal or plasmidic location of the cpe gene, respectively. Clostridial pathogenicity has been correlated to protease and azoreductase production.

Pulsed field, PCR ribotyping and multiplex PCR analysis of Yersinia enterocolitica strains isolated from meat food in San Luis Argentina.

The characterization of phenotypic and genotypic virulence markers of Yersinia enterocolitica strains belonging to biotypes (B) 1A, 2 and 3, mostly isolated from food in San Luis, Argentina, and the assessment of their genotypic diversity using PFGE and PCR ribotyping, were performed in our laboratory for the first time. Thirty five Y. enterocolitica strains, two reference strains and 33 strains isolated in our laboratory were studied.

Prevalence of some bacteria yeasts and molds in meat foods in San Luis, Argentina.

In this work we evaluate the microbiological quality and the hygiene degree of meat foods consumed in the city of San Luis. A total of 515 meat food samples (315 from fresh sausages, 100 from hamburgers and 100 from ground beef) were processed, being the most of them non-industrial products. The microbiological quality was determined by counts of total mesophilic bacteria, coliforms, Escherichia coli, molds and yeasts, and Clostridium perfringens.

Correlation between Yersinia enterocolitica and type I collagen reactivity in patients with arthropathies.

We investigated the association with Yersinia infection in patients with arthropathies in our region. To assess the reactivity to articular antigens, the correlation of anti-Yersinia with anti-type I and type II collagen antibodies was studied. Sera from 124 patients with musculoskeletal symptoms, and 47 synovial fluids (SF) from patients with rheumatoid arthritis (RA), spondyloarthopathies (SpA) or osteoarthritis (OA) were examined.

Prevalence and characterization of Clostridium perfringens from spices in Argentina.

Spices can present high microbial counts and Clostridium perfringens, Bacillus cereus, Salmonella and Shigella, among others have been isolated from spices. C. perfringens is an important pathogen agent causing, among other diseases, enteritis in humans caused by C.

Genotypic and phenotypic characteristics of Yersinia enterocolitica isolated from the surface of chicken eggshells obtained in Argentina.

The prevalence of Yersinia enterocolitica on chicken eggshell surfaces in San Luis, Argentina, was investigated. The pathogenic potential of recovered isolates was assessed by means of phenotypic virulence tests and the presence of the 72-kb pYV plasmid. Antimicrobial susceptibility was determined by the agar diffusion method.

Immunogenic protein variations of Clostridium chauvoei cellular antigens associated with the culture growth phase.

The immunoprotective capacity of four Clostridium chauvoei strains at different growth stages is reported. In all the strains tested, the cells coming from the stationary phase were those with the highest immunoprotective capacity and, depending on the strain, this protective capacity diminished or even disappeared in other phases. Protein profiles were similar in all the strains and few proteins were differentially expressed during growth as shown by SDS-PAGE.

Survival of Aeromonas hydrophila in Fresh Tomatoes ( Lycopersicum esculentum Mili) Stored at Different Temperatures and Treated with Chlorine.

This study examines the survival of two Aeromonas hydrophila strains ( A. hydrophila ATCC 7965 [strain A] and A. hydrophila isolated from food [strain B]) on the surface and core tissue of fresh tomatoes stored at different temperatures and the efficacy of chlorine treatment on their survival.

Prevalence of Yersinia enterocolitica in Hake (Merluccius hubbsi) Fillets.

Yersinia enterocolitica is a human pathogenic bacterium. The prevalence of Y. enterocolitica in refrigerated hake fillets sold for human consumption in retail stores was investigated in order to determine the degree of pathogenicity.

Biovars, Serovars, and Phagovars of Yersinia enterocolitica Isolated From 450 Samples of Cold Food in San Luis, Argentina.

A search of Yersinia enterocolitica in foods of animal origin has been carried out. Isolates were obtained from 450 samples of cold foods: 100 samples of cooked ham, 150 samples of salami, 100 samples of porcine cheese (artisan cold foods), and 100 samples of mortadella. Enrichments were performed in 0.

Enterotoxin Production in Two-Culture Media by Yersinia enterocolitica Isolated from Sausages, Porcine Ceca and Tongues in San Luis, Argentina.

Twenty-six strains of Yersinia enterocolitica isolated from fresh sausages, bovine tongues, and porcine ceca and tongues were evaluated for the production of heat-stable enterotoxin (ST, infant mouse test) in trypticase soy broth (TSB) and casamino acids broth (CAB). The gut weight/body weight ratios obtained with the two media were similar. Sixteen strains produced ST in both media; nine strains did not.

Incidence of Clostridium perfringens in Fresh Sausages in Argentina.

A simple iron-milk medium was used for the isolation and enumeration (MPN) if Clostridium perfringens from fresh sausages. Samples were diluted (log 10) in 0.1% peptone water, aliquots seeded and incubated at 45°C for 16-18 h, and cultures with clots and stormy fermentation were considered positive.