ST471 Producing VIM-4 Metallo-β-Lactamase in Colombia.

An isolate sequence-type 471 (ST471) producing Verona integron-encoded metallo-β-lactamases (VIM)-4 was recovered from a rectal swab in a patient without travel records with osteomyelitis in Colombia. The isolate carried a class 1 integron-borne gene with a 170-bp duplication in the 3' end of the gene, preceded by an ')-Ib gene. The genetic environment of , , and genes showed similarities to the backbone of pKKp4, an IncA/C-type plasmid from a strain carrying recovered in Kuwait.

Genetic Diversity of Multidrug-Resistant Isolates Carrying and Genes That Spread on Different Genetic Environment in Colombia.

is an opportunistic Gram-negative pathogen with an increase in the frequency of infections caused by multidrug resistant (MDR) and extensively drug resistant (XDR) strains, limiting the available therapeutic options. The most troublesome resistance is the acquisition and production of carbapenemases such as Verona integron-encoded metallo-β-lactamases (VIM), the most frequent and widespread, and the carbapenemases (KPC), which has continuously spread in the last decade. Its dissemination is linked to their location on mobile genetic elements (MGEs).

Dynamics of Dissemination from Non-CG258 to Other via IncN Plasmids in an Area of High Endemicity.

Carbapenem-resistant (CRE) pose a significant threat to global public health. The most important mechanism for carbapenem resistance is the production of carbapenemases. carbapenemase (KPC) represents one of the main carbapenemases worldwide.

Distribution and molecular characterization of beta-lactamases in Gram-negative bacteria in Colombia, 2001-2016.

Beta-lactamases are enzymes with hydrolytic activity over beta-lactam antibiotics and they are the main resistance mechanism in Gram-negative bacteria. Extended-spectrum beta-lactamases (ESBL), AmpC, and carbapenemases have the greatest clinical and epidemiological impact in hospital settings. The increasing frequency and worldwide spread of these enzymes have limited the therapeutic options in hospital-acquired infections and those originating in the community.

[Successful in vitro culture of Plasmodium falciparum gametocytes].

Introduction: The sexual stages of Plasmodium falciparum have not been studied in as much detail as the asexual stages due to the lack of standardized in vitro cultures as well as difficulties in identifying the sexual development stages of the parasite. These difficulties hamper the studies on biology, metabolism, gene expression and protein synthesis during sexual stages. Each of these facets are important targets in antimalarial drug research, particularly the identification of potential therapeutic agents against Plasmodium (derived mainly from plants).