Electrosynthesis of N-Methylisatin.

Isatin in a solution of dry N,N-dimethylformamide/NaClO is electroreduced in the presence of CHI. N-methylisatin (NMI) is obtained in quantitative molar yield and high current efficiency by controlled potential electrolysis (CPE). NMI and N-methylisatoic anhydride are the reaction products when CPE is performed in the absence of CHI, but adding it once the CPE was completed.

Detergent resistant domains in erythrocyte membranes survive after cell cholesterol depletion: an EPR spin label study.

We use electron paramagnetic resonance (EPR) with liposoluble spin labels in order to study the lipid structures obtained after Triton X-100 extraction of erythrocyte membranes. The apparent order profile in these detergent resistant membranes (DRM) is very similar to that of the parent membrane, although with higher absolute values, consistent with a liquid-ordered state. DRM could also be obtained from erythrocytes previously depleted in a 40% of their membrane cholesterol, in apparent opposition to the phenomenon of raft disruption reported by other authors.