The role of kinins in the proliferation of fibroblast primed with TNF in scratch wound assay: Kinins and cell proliferation.

The aim of this study was to evaluate the involvement of both B and B kinins receptors (BR and BR) in the fibroblast proliferation induced by the cytokine tumour necrosis factor (TNF) attempting to establish an in vitro model of wound healing. Murine fibroblasts L-929 were cultivated in 24 wells plaque until total confluence (DMEM (Vitrocell®); 5% fetal bovine serum, 5% CO, 37 °C) and then submitted to the scratch assay. The cells were treated with PBS, TNF (2 ng/mL) and/or mr-TNF antibody (200 μg/mL), or PDTC.