Publications by authors named "Ana J Caillava"
Article Synopsis
- - Chikungunya fever, caused by the chikungunya virus (CHIKV), leads to acute arthritis that may develop into chronic pain, with a recently approved vaccine not widely accessible in endemic areas.
- - A new vectored vaccine using baculovirus technology shows promise by presenting CHIKV proteins E1 and E2 on its surface, eliciting strong immune responses in tested mice, including the production of neutralizing antibodies.
- - Mice receiving two doses of this baculovirus-based vaccine were fully protected from CHIKV infection, highlighting its potential as an effective vaccine platform against this re-emerging disease amidst its surge in cases, particularly in the Americas.
Article Synopsis
- Bovine brucellosis is a significant zoonotic disease that affects cattle and poses economic and public health challenges, highlighting the need for effective diagnostic tools.
- A new immunochromatographic test, called Brucella GlycoStrip, was developed to detect IgG antibodies in bovine serum and showed high sensitivity and specificity (96.9% and 100%, respectively) for diagnosing the disease.
- This rapid (10-minute) test can differentiate between infected and vaccinated animals, making it a valuable tool for controlling bovine brucellosis in field settings.
In the last decades bacterial glycoengineering emerged as a new field as the result of the ability to transfer the Campylobacter jejuni N- glycosylation machinery into Escherichia coli for the production of recombinant glycoproteins that can be used as antigens for diagnosis, vaccines, and therapeutics. However, the identification of critical parameters implicated in the production process and its optimization to jump to a productive scale is still required. In this study, we developed a dual expression glycosylation vector for the production of the recombinant glycoprotein AcrA-O157, a novel antigen that allows the serodiagnosis of the infection with enterohemorrhagic E.
View Article and Find Full Text PDFBrucellosis is a highly zoonotic disease that affects animals and human beings. Brucella suis is the etiological agent of porcine brucellosis and one of the major human brucellosis pathogens. Laboratory diagnosis of porcine brucellosis mainly relies on serological tests, and it has been widely demonstrated that serological assays based on the detection of anti O-polysaccharide antibodies are the most sensitive tests.
View Article and Find Full Text PDFArticle Synopsis
- Access to effective diagnostic tools is crucial for improving global health and timely detection of infectious agents for early treatment.
- Conventional methods for detecting pathogens are often slow, complex, and expensive, making them unsuitable for use in resource-limited settings.
- The newly developed electrochemical magnetic microbeads-based biosensor (EMBIA) offers a portable, cost-effective solution for point-of-care diagnosis of various infectious diseases, showing diagnostic performance comparable to traditional methods.
Article Synopsis
- Shiga toxin-producing E. coli (STEC) is a significant cause of hemolytic-uremic syndrome (HUS), leading to serious health issues like anemia and kidney failure, with E. coli O157:H7 being the most common type globally.
- The study utilized a new bacterial glycoengineering technique to create specific glycoproteins for diagnosing STEC-associated HUS, which improved detection accuracy compared to traditional methods.
- The findings showed that these new diagnostic tools could identify infections early and effectively match serotypes with isolated strains, highlighting their potential for clinical use in diagnosing and managing HUS patients.