Clinical evaluation of combined autologous Platelet-Rich Plasma and Volume-Controlled Ozone Therapy in Facial Rejuvenation: A randomized controlled Pilot Study.

Platelet rich plasma and ozone therapy have been suggested in the clinical setting as promising and adjuvant therapies for managing the symptoms related to the facial aging. However, there is a lack of evidence about the effects of combining both therapies on rejuvenation. Thus, the aim of the present study was to evaluate and to compare the safety and efficacy of both therapies in facial aging.

Comparison of Different Methods for Spongin-like Collagen Extraction from Marine Sponges ( and ): Physicochemical Properties and In Vitro Biological Analysis.

This study aimed to compare different protocols (Protocol 1: P1; Protocol 2: P2; Protocol 3: P3; Protocol 4: P4) for the extraction of spongin-like collagen (SC) from marine sponges. The SEM micrographs demonstrated a fibrillar structure for the extracts from and the nodular/particulate aggregates for . FTIR showed for all samples peaks similar to collagen for both species.

Alendronate release from calcium phosphate cement for bone regeneration in osteoporotic conditions.

Osteoporosis represents a major health problem in terms of compromising bone strength and increasing the risk of bone fractures. It can be medically treated with bisphosphonates, which act systemically upon oral or venous administration. Further, bone regenerative treatments in osteoporotic conditions present a challenge.

Effects of 660 nm low-level laser therapy on muscle healing process after cryolesion.

The aim of this study was to evaluate the effects of 660 nm low-level laser therapy (LLLT) on muscle regeneration after cryolesion in rat tibialis anterior muscle. Sixty-three Wistar rats were divided into a control group, 10 J/cm(2) laser-treated group, and 50 J/cm(2) laser-treated group. Each group formed three subgroups (n = 7 per group), and the animals were sacrificed 7, 14, or 21 d after lesion.

Characterization and in vivo biological performance of biosilicate.

After an introduction showing the growing interest in glasses and glass-ceramics as biomaterials used for bone healing, we describe a new biomaterial named Biosilicate. Biosilicate is the designation of a group of fully crystallized glass-ceramics of the Na₂O-CaO-SiO₂-P₂O₅ system. Several in vitro tests have shown that Biosilicate is a very active biomaterial and that the HCA layer is formed in less than 24 hours of exposure to "simulated body fluid" (SBF) solution.

The effects of 780-nm low-level laser therapy on muscle healing process after cryolesion.

The objective of this study was to assess the effects of 780-nm low-level laser therapy at different periods of 7, 14 and 21 days after cryolesion, including the dose (10 or 50 J/cm(2)), to promote a better muscle repair evidenced by histopathological and immunohistochemical analyses. Fifty-four male rats were divided into three groups: injured control group (CG)-injured animals without any treatment; injured 780-nm laser-treated group, at 10 J/cm(2) (G10); and injured 780-nm laser-treated group, at 50 J/cm(2) (G50). Each group was divided into three subgroups (n = 6): 7, 14 and 21 days post-injury.

Histopathological, cytotoxicity and genotoxicity evaluation of Biosilicate® glass-ceramic scaffolds.

This study evaluated the biocompatibility of Biosilicate® scaffolds by means of histopathological, cytotoxicity, and genotoxicity analysis. The histopathologic analysis of the biomaterial was performed using 65 male rats, distributed into the groups: control and Biosilicate®, evaluated at 7, 15, 30, 45, and 60 days after implantation. The cytotoxicity analysis was performed by the methyl thiazolyl tetrazolium (MTT) assay, with various concentrations of extracts from the biomaterial in culture of osteoblasts and fibroblasts after 24, 72, and 120 h.

Effects of biosilicate and bioglass 45S5 on tibial bone consolidation on rats: a biomechanical and a histological study.

The purpose of this study was to investigate the effects of Bioglass 45S5 and Biosilicate, on bone defects inflicted on the tibia of rats. Fifty male Wistar rats were used in this study, and divided into five groups, including a control group, to test Biosilicate and Bioglass materials of two different particle sizes (180-212 microm or 300-355 microm). All animals were sacrificed 15 days after surgery.

Effects of a progressive loading exercise program on the bone and skeletal muscle properties of female osteopenic rats.

The aim of this study was to determine the effects of a progressive loading exercise program on the bone and skeletal muscle properties of female osteopenic rats. Forty female Wistar rats (12 weeks old) were divided into four groups (n=10): sham-operated sedentary (SS); osteopenic-sedentary (OS); sham-operated training (ST) and osteopenic training (OT). Osteopenia was induced by ovariectomy (OVX).