Reliable and sensitive analytical platform to assess dietary exposure of pigs to mycotoxins and explore potential urinary biomarkers.

A reliable and sensitive analytical platform is proposed for the assessment of pig exposure to mycotoxins through the consumption of commercial feed. A total of 48 naturally contaminated feed and 55 urine samples collected from eight Spanish farms were analyzed using a fast and simple methodology based on solid-liquid extraction (SLE) or liquid-liquid extraction (LLE) and dispersive liquid-liquid microextraction (DLLME). High-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) was used for the targeted analysis of 27 mycotoxins from different families in both matrices achieving limits of quantification in a range of 0.

Evaluation of distribution of emerging mycotoxins in human tissues: applications of dispersive liquid-liquid microextraction and liquid chromatography-mass spectrometry.

In this work, a complete study of the distribution of emerging mycotoxins in the human body has been carried out. Specifically, the presence of enniatins (A, A, B, B) and beauvericin has been monitored in brain, lung, kidney, fat, liver, and heart samples. A unique methodology based on solid-liquid extraction (SLE) followed by dispersive liquid-liquid microextraction (DLLME) was proposed for the six different matrices.

Headspace with Gas Chromatography-Mass Spectrometry for the Use of Volatile Organic Compound Profile in Botanical Origin Authentication of Honey.

The botanical origin of honey determines its composition and hence properties and product quality. As a highly valued food product worldwide, assurance of the authenticity of honey is required to prevent potential fraud. In this work, the characterisation of Spanish honeys from 11 different botanical origins was carried out by headspace gas chromatography coupled with mass spectrometry (HS-GC-MS).

Toward Nitrite-Free Curing: Evaluation of a New Approach to Distinguish Real Uncured Meat from Cured Meat Made with Nitrite.

After the International Agency for Research on Cancer (IARC) classified ingested nitrites and nitrates as "probably carcinogenic to humans" under conditions favoring endogenous nitrosation, several meat products labeled as "made without nitrite" were launched. In order to distinguish uncured products truly made without nitrite from cured products made with any nitrite source (vegetal or mineral), this article presents an approach to detect and quantify nitrite from different origins added to meat. The method consists on the determination of nitrous oxide as a target compound using headspace gas chromatography-mass spectrometry (HS-GC-MS).

Untargeted headspace gas chromatography - Ion mobility spectrometry analysis for detection of adulterated honey.

The recognized properties of honey together with its price have, almost inevitably, led to economically motivated adulteration. In this work, headspace gas chromatography coupled to ion mobility spectrometry (HS-GC-IMS) is proposed for the differentiation of honey according to its purity and the level of adulteration by sugar cane or corn syrups. An easy and rapid sample treatment, consisting of incubating 1 g of honey at 100 °C for 15 min and then injecting 750 μL of the sample headspace into the GC-IMS system, is proposed.