Publications by authors named "Ana C B Araujo"
Functional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized.
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- Lytic polysaccharides monooxygenases (LPMOs) challenge traditional views on cellulose breakdown by enabling oxidative cleavage alongside hydrolases, enhancing biomass conversion for biofuels and chemicals.
- The E7 LPMO from Thermobifida fusca was successfully expressed in both E. coli and Pichia pastoris, allowing for biochemical analysis of its activity on cellulose substrates.
- Mass spectrometry revealed that the E7 LPMO effectively cleaves cellulose, but reaction optimization is needed to improve cellulose breakdown efficiency.
The environmental quality of the Jacuípe River's estuary (very important in northeastern Brazil) was assessed during 2007 and 2008. In water, concentrations (mg L⁻¹) of NO₂⁻ (<0.004 to 0.
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