[Emergency counterplan for drinking water pollution accident].

After the almost nation-wide outbreak of SARS this year, all the health administration agencies have made attempt to analyze the origin of the epidemic and draw lessons from the measures taken against it, hoping to be better able to cope with such potential public health threats. For effectively preventing and timely controlling drinking water pollution accident so as to ensure the sanitation of life drinking water, it is of great importance to prepare emergency counterplan beforehand against such accident. In this article the author proposes the emergency counterplan against urban drinking water pollution on the basis of established laws and regulations of the state and Beijing municipality.

[Expression of recombinant human endostatin in Pichia pastoris and its inhibitory effects on the growth of human lungadenocarcinoma Astc-a-1 cells].

Objective: To achieve expression of recombinant human endostatin (rhES) in a highly efficient foreign gene expression system, the yeast strain Pichia pastoris, and to evaluate the inhibitory effect of rhES on the growth of nude mouse pulmonary adenocarcinoma cells.

Methods And Results: The rhES gene was efficiently expressed in the yeast strain, and heparin affinity chromatography yielded highly purified endostatin identified by Western blotting, which proved to significantly inhibit the growth of the ECV-304 cells in vitro and also the growth of the lung adenocarcinoma Astc-a-1 in nude mice.

Conclusions: The rhES gene can be expressed in Pichia pastoris, and has the ability to restrain the growth of ECV-304 cells and lung adenocarcinoma Astc-a-1 in nude mice, showing important potentials for future clinical applications.