[Preparation and functional identification of testicular Sertoli cells].

Objective: To simplify the method for separation and cultivation of rat testicular Sertoli cells with high viability, quantity and expression efficiency.

Methods: Testicular Sertoli cells from 2 to 3-week-old male Wistar rats were prepared by digestion with collagenase, trypsin and DNase and cultured together with active lymphocytes to observe their killing effect against lymphocytes. After cell culture for 72 h, the Sertoli cells were morphologically observed by different means and identified with transmission electron microscope.