Expression and significance of P53 protein and MDM-2 protein in human gliomas.

Background: P53 is one of the most studied tumor suppressors in the cancer research, and over 50% of human tumors carry P53 mutations. MDM-2 is amplified and/or overexpressed in a variety of human tumors of diverse tissue origin. The aim of this study was to examine the expression of P53 protein and MDM-2 protein in gliomas, and to investigate the relationship between the expression of the two proteins and the histopathological grades of glioma.

[Clinicopathologic evaluation of 36 cases of allergic fungal sinusitis].

Objective: To characterize clinicopathological features of allergic fungal sinusitis (AFS).

Methods: Thirty-six cases of AFS were retrieved from the department archival files of Beijing Tongren Hospital from 2002 to 2006. AB-PAS, GMS and MUC5B stain were performed using paraffin-embedded tissues of the cases.

Increased expression of fascin associated with malignant transformation of sinonasal inverted papilloma.

Background: Fascin, an actin binding protein, usually expressed at a low level in normal epithelium, but is significantly increased in transformed epithelial cells and several common carcinomas. In this study, we examined the expression of fascin by immunohistochemistry in sinonasal epithelium with chronic inflammation (control group), exophytic papilloma (EP), inverted papilloma (IP) with dysplasia and cancerated IP (including carcinoma in situ and invasive squamous cell carcinoma, SCC), and furthermore investigated the relationship between fascin expression and formation of malignant IP.

Methods: Fascin expression was immunohistochemically detected using monoclonal antibody against fascin in 86 paraffin embedded tissues, including 10 cases of sinonasal mucosa with chronic inflammation, 10 of EP, 45 of IP with dysplasia (45 cases were divided into three groups: IP with mild dysplasia, IP with moderate dysplasia, and IP with severe dysplasia, 15 cases each), and 21 of cancerated IP.

[The change of circulating endothelial cell in blood of rats and pulmonary small artery vascular endothelial cell under acute hypoxia].

Aim: To investigate changes of VEC and CEC under acute hypoxia.

Methods: Observe CEC in blood under acute hypoxia morphologically and count the number of CEC by optical microscope, measure LDH activity of young CEC and VEC by histochemical staining image analysis.

Results: LDH activities of VEC in hypoxic groups are lower than that in the group before hypoxia and decrease progressively with hypoxia time.