Long non-coding RNA SOX21-AS1 sponges miR-145 to promote the tumorigenesis of colorectal cancer by targeting MYO6.

Emerging evidences have proved that long non-coding RNAs (lncRNAs) act as important molecular regulator in the tumor progression, including colorectal cancer (CRC). LncRNA SOX21-AS1 has been verified as oncogenic molecular in other cancers and tumorigenesis. In present study, our team investigates the clinical characteristic and molecular function in CRC carcinogenesis.

[Cluster analysis of smoking, alcohol drinking and other health risk behaviors in undergraduate students].

Objective: To investigate the status of smoking and alcohol drinking behaviors in undergraduate students, and explore the relationship between smoking and alcohol drinking and other health risk behaviors.

Methods: A total of 7 979 students from 44 universities or colleges across China were sampled with multiple-stage stratified sampling method. A cross-sectional investigation on smoking, alcohol drinking and other health risk behaviors was conducted, and SPSS 13.

Stimulation of dendritic cell maturation and induction of apoptosis in leukemia cells by a heat-stable extract from azuki bean (Vigna angularis), a promising immunopotentiating food and dietary supplement for cancer prevention.

Non-toxic stimulation of dendritic cells (DCs), which are central immunomodulators, may aid the prevention of cancer. Furthermore, induction of apoptosis in cancer cells by anticancer agents contributes to the induction of DC maturation. We previously reported that extracts from Pinus parviflora Sieb.

[A cross-sectional study on health-related knowledge and skills and its influencing factors among the Chinese college students].

Objective: To investigate the status on health-related knowledge and skills among the college students.

Methods: 5070 Chinese students from 28 universities or colleges were sampled, using the multiple-stage stratified, purposive and convenient mixed sampling method. A cross-sectional investigation on health literacy was conducted with self-designed questionnaire, and SPSS 13.

Barley low molecular weight β-glucan potently induces maturation of mouse dendritic cells.

Background: Accumulating evidence indicates that non-toxic immunostimulants with strong differentiation/maturation-inducing activity for dendritic cells (DCs) might be useful for preventing or even curing cancer.

Materials And Methods: Mouse bone marrow (BM) cells were cultured in the presence of various glucans and their differentiation/maturation-inducing activities were compared by measuring cytokines secreted in the culture medium.

Results: Barley-derived β-glucan with an average molecular weight of 2 kDa (BBG-Low) remarkably stimulated the formation of mature DCs from immature mouse DCs.

A heat-stable extract from Mucuna stimulates the differentiation of bone marrow cells into dendritic cells and induces apoptosis in cancer cells.

Pine cone extract is known to induce differentiation of human mononuclear cells into dendritic cells (DCs) and also to induce apoptosis in human cancer cells. In the present study, we screened edible plants that contain components with biological activities similar to or more potent than those of pine cone extract. We found that Mucuna (Mucuna pruviens var.

[A preliminary study on the occlusal contact changes during retention in adolescent patients].

Objective: To evaluate the changes of the occlusal contact characteristics in adolescent patients during 12 months after active orthodontic treatment.

Methods: Twenty adolescent patients with Hawley retainers after active orthodontic treatment were divided into none occlusal interference group and occlusal interference group. The occlusion of the patients was examined with T-Scan II system directly after the appliance removal (T1) and after an average retention period of 12 months (T2).

Dendritic cell differentiation and tumor cell apoptosis induced by components of a poly-phenylpropanoid polysaccharide complex.

Background: As part of the indigenous folk medicine in Japan, aqueous extracts of pine cones have been used for over a century to treat cancer and other illnesses and references to their use can be found in ancient Greek literature. However, the mechanisms by which such extracts work are largely unknown.

Materials And Methods: Murine bone marrow (BM)-derived dendritic cells (DCs) and human monocyte U937 cells were treated in vitro with an extract prepared from pine cones (termed poly-phenylpropanoid polysaccharide complex, PPC).

[Occlusal contacts in intercuspal position after orthodontic treatment].

Objective: To observe the occlusal contacts in intercuspal position(ICP) in adolescent patients after orthodontic treatment.

Methods: Fourteen adolescent patients after orthodontic treatment were selected as experimental group and 17 healthy adolescents and young adults with normal occlusion were selected as control group. The occlusal contact in ICP in the two groups were recorded with T-Scan II system and compared.

[Genetic diversity of different geographical populations of Oxya chinensis based on AFLP analysis].

In order to study the genetic diversity and genetic structure of populations, 7 populations of Oxya chinensis from 7 provinces (or cities) of China were analyzed using AFLP technique. A total of 336 reproducible bands were amplified with 7 primer combinations from 128 individuals. Two hundred and ninety-two bands (86.

Mitogen-activated protein kinase-dependent apoptosis in norcan-tharidin-treated A375-S2 cells is proceeded by the activation of protein kinase C.

Background: We have reported that norcantharidin (NCTD) induces human melanoma A375-S2 cell apoptosis and that the activation of caspase and the mitochondrial pathway are involved in the apoptotic process. This study aimed at investigating the roles of mitogen-activated protein kinase (MAPK) and protein kinase C (PKC) in A375-S2 cell apoptosis induced by NCTD.

Methods: We assessed the effects of NCTD on cell growth inhibition using the 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheyltetrazolium bromide (MTT) assay, DNA fragmentation (DNA agarose gel electrophoresis), and MAPK protein levels (Western blot analysis) in A375-S2 cells.

Norcantharidin induces apoptosis in HeLa cells through caspase, MAPK, and mitochondrial pathways.

Aim: To investigate the mechanism of norcantharidin (NCTD)-induced HeLa cell apoptosis.

Methods: HeLa cell growth inhibition was measured by MTT method. Apoptosis was detected by Hoechst 33258 staining and agarose gel electrophoresis.

Norcantharidin induces human melanoma A375-S2 cell apoptosis through mitochondrial and caspase pathways.

Norcantharidin (NCTD) is the demethylated form of cantharidin, which is the active substance of mylabris. To examine the pathway of NCTD-induced A375-S2 cell death, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-dipheyltetrazolium bromide (MTT) assay, photomicroscopical observation, DNA agarose gel electrophoresis, caspase activity assay and Western blot analysis were carried out. A375-S2 cells treated with NCTD exhibited several typical characteristics of apoptosis.