Diagnostic value of stool DNA testing for multiple markers of colorectal cancer and advanced adenoma: a meta-analysis.

Background And Objectives: The diagnostic value of stool DNA (sDNA) testing for colorectal neoplasms remains controversial. To compensate for the lack of large-scale unbiased population studies, a meta-analysis was performed to evaluate the diagnostic value of sDNA testing for multiple markers of colorectal cancer (CRC) and advanced adenoma.

Methods: The PubMed, Science Direct, Biosis Review, Cochrane Library and Embase databases were systematically searched in January 2012 without time restriction.

High-resolution melting assay (HRMA) is a simple and sensitive stool-based DNA Test for the detection of mutations in colorectal neoplasms.

Background: Stool-based DNA testing for colorectal cancer is becoming a favored alternative to existing DNA screening tests. However, current methods of analysis often become more complicated and costly with increased sensitivity. The high-resolution melting assay (HRMA) is a simple and rapid mutation scanning method with low cost and superb accuracy.

Is high resolution melting analysis (HRMA) accurate for detection of human disease-associated mutations? A meta analysis.

Background: High Resolution Melting Analysis (HRMA) is becoming the preferred method for mutation detection. However, its accuracy in the individual clinical diagnostic setting is variable. To assess the diagnostic accuracy of HRMA for human mutations in comparison to DNA sequencing in different routine clinical settings, we have conducted a meta-analysis of published reports.

[Screening of high-risk group with colorectal cancer].

Objective: To measure the rate of high-risk group and the detection rate of colorectal cancer (CRC) in communities in Guangdong province and to provide scientific rationales for formulating mass screening plans in high-risk group.

Methods: Mass survey was conducted by questionnaire combined fecal occult blood test (FOBT) in Huizhou region, Guangdong Province, to sort out the high-risk population of CRC. Then the high-risk population was screened by colonoscopy and pathology to identify CRC.

[Epidemiology investigation of colorectal cancer on community group in Guangdong province].

Objective: To explore the epidemiology characteristics of colorectal cancer on community group in Guangdong province.

Methods: Huidong was took as the research spot to investigate the sickness and incidence of colorectal cancer among a whole community group in Guangdong province, and two projects were used simultaneously: "screening of colorectal cancer in high risk group" and "screening project using fecal occult blood test". The numeration time for population of 10 towns was set at July 1, 2005.

[Effect of Tiam1 overexpression on proliferation and metastatic potential of human colorectal cancer].

Objective: To confirm the role of Tiam1 (T lymphoma invasion and metastasis 1) gene in the proliferation and metastasis of colorectal cancer.

Methods: Proliferative and metastatic abilities of Tiam1 transfectant were investigated by subcutaneous injection of cells and surgical orthotopic transplantation (SOI) in mice.

Results: The expression of Tiam1 led to a pronounced increase in HT29/Tiam1 cell growth starting from day 7, up to 2.

[The trend of clinical characteristics of colorectal cancer during the past 20 years in Guangdong province].

Objective: To study the trend of clinical characteristics of colorectal cancer during the past 20 years in Gaungdong province.

Methods: The clinical data of 3870 cases of colorectal cancers confirmed pathologically who were hospitalized to Nanfang Hospital and Huizhou Municipal Hospital, Guangdong province, 1985 - 2004 were divided into 5-year groups and analyzed.

Results: The hospitalization number of colorectal cancer in the 2000 - 20004 5-year period was 102%, higher than that of the 1985 - 1989 period with an annual increase of 5.

[The risk factors and impact of gastroesophageal reflux disease on quality of life in general population].

Objectives: To assess the risk factors of gastroesophageal reflux disease (GERD) in the general population and its impact on health-related quality of life (HRQOL).

Methods: A face-to-face interview was carried out in Guangdong Province using a validated Chinese version Reflux Disease Questionnaire (RDQ) to assess the prevalence of GERD. Random clustered sampling of permanent inhabitants aged 18 to 90 years was carried out under stratification of urban and suburban areas.

[Clinical epidemiological characteristics of 3870 cases of colorectal cancers in Guangdong region].

Objective: To explore the clinical characteristics and pattern of incidence of colorectal cancers in Guangdong region.

Methods: Analysis and summary were made for 3870 colorectal cancer patients pathologically confirmed in the Nanfang Hospital and Huizhou Municipal Central Hospital of Guangdong province.

Results: Median age of 3870 cases with colorectal cancer was 55.

[Tiam1 expression correlates with the biological behavior of human colorectal carcinoma cells].

Objective: To investigate whether the Tiam1 gene expression enhances the invasive and metastatic capabilities of colorectal carcinoma cells.

Methods: Endogenous expression of Tiam1 in five colorectal carcinoma cell lines was investigated by RT-PCR. Tiam1/C1199HA cDNA was transfected into HT29, a colorectal carcinoma cell line without endogenous Tiam1 expression.

[Evaluation of the quality of life of patients with irritable bowel syndrome].

Objective: To explore and assess the health-related quality of life (HRQOL) of irritable bowel syndrome (IBS) patients in the population.

Methods: Random clustered sampling involving permanent inhabitants aged 18 - 80 yr was carried out under stratification of urban and suburban areas in Guangdong Province. Altogether 231 IBS patients fulfilling the Rome II criteria and 636 Non-IBS as control were collected.

[A population-based epidemiologic study of irritable bowel syndrome in Guangdong province].

Objective: To explore the prevalence of irritable bowel syndrome (IBS) and its risk factors in Guangdong province.

Methods: A questionnaire was used to screen IBS by face-to-face interviews according to Manning (modified including symptoms of constipation) and Rome II criteria. Random clustered sampling involving permanent inhabitants aged 18 - 80 years was carried out under stratification of urban and suburban areas.