SARS-CoV-2 Structural Proteins Modulated Blood-Testis Barrier-Related Proteins through Autophagy in the Primary Sertoli Cells.

The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) disrupts the blood-testis barrier (BTB), resulting in alterations in spermatogenesis. However, whether BTB-related proteins (such as ZO-1, claudin11, N-cadherin, and CX43) are targeted by SARS-CoV-2 remains to be clarified. BTB is a physical barrier between the blood vessels and the seminiferous tubules of the animal testis, and it is one of the tightest blood-tissue barriers in the mammalian body.

Protective effects of dietary betaine on intestinal barrier function and cecal microbial community in indigenous broiler chickens exposed to high temperature environment.

High temperature environment causes reduction in productivity in broilers by disrupting the intestinal barrier function. This study aimed to investigate the protective effects of dietary betaine on intestinal barrier function and cecal microbial community in indigenous broilers (Huaixiang chickens) exposed to high temperature environment. A total of 144 5-week-old male broilers (average initial body weight of 401.

New insights into the role of dietary marine-derived polysaccharides on productive performance, egg quality, antioxidant capacity, and jejunal morphology in late-phase laying hens.

The present study was conducted to evaluate the effects of dietary marine-derived polysaccharides (MDP) from seaweed Enteromorpha on productive performance, egg quality, antioxidant capacity, and jejunal morphology in late-phase laying hens. A total of 240 Lohmann white laying hens (62 wk of age) were assigned to 4 dietary treatments that included MDP at concentrations of 0, 1,000, 2,500, and 5,000 mg/kg for 6 wk. Each treatment had 6 replicates with 5 cages (2 birds/cage).

Sodium butyrate as an effective feed additive to improve growth performance and gastrointestinal development in broilers.

This study was conducted to evaluate the effects of dietary sodium butyrate (SB) supplementation on growth performance, the development of gastrointestinal tract and immune organs (thymus, spleen and bursa of fabricius), and serum antibody titer after Newcastle disease (ND) vaccination in broilers. The total of 288 1-day-old broilers were randomly allocated to four groups with six replications according to initial body weight. Four treatment groups were designed as follows and fed the indicated diets: CON, basal diet; T1, basal diet supplemented with 0.

Chicken GHR antisense transcript regulates its sense transcript in hepatocytes.

An increasing number of evidences indicated that long noncoding RNAs (LncRNAs) regulate a variety of biological progresses via different mechanisms. Our previous study had identified a chicken growth hormone receptor (GHR) antisense transcript (GHR-AS) which regulated GHR sense transcript (GHR-S) in LMH cells. In the present study, roles of GHR-AS and its regulatory mechanism were analyzed in chicken hepatocytes.

A genetics laboratory class to analyze early and late feather traits of chicken.

With the implementation of the "Education and Training Program for Outstanding Agricultural and Forestry Talents" in our country, our university established the "Outstanding Class" for students majoring in the animal science. We also carried out a series of educational management and curriculum reforms to cultivate students' systematic model of thinking and the ability of technology innovation. In this paper, we designed a comprehensive experiment that focused on analyzing early and late feather genetic traits of chicken.

Selection of reference genes for gene expression studies in PBMC from Bama miniature pig under heat stress.

Heat stress decreases immune function and increases disease susceptibility in stressed animals, which are important factors for industry and public health. We investigated the molecular mechanisms underlying heat stress by profiling the expression of target genes involved in the cellular response in the blood of Bama miniature pigs (Sus scrofa domestica) over 21 days with the use of quantitative RT-PCR (qRT-PCR). Reliable standards were established for the normalization of qRT-PCR.

[Research on differentiation features of the monoclonal human pancreatic stem cell].

The in vitro and in vivo differentiation features of the monoclonal human pancreatic stem cell (mhPSC) line derived from the pancreatic tissues of a male abortive fetus at 4 month-old were studied. The mhPSCs were plated in culture dishes that had been coated with 0.1% gelatin in phosphate-buffered saline without calcium and magnesium.

[Establishment of the isolation and culture system for monocloning human pancreatic stem cell].

The present study aimed to establish an isolation and culture system for the monoclonal human pancreatic stem cells and monoclonal human pancreatic stem cell line. Some factors which would influence the proliferation of the monoclonal human pancreatic stem cells were assessed. Pancreatic tissues, taken from abortive fetuses by sterile procedures, were dissected into 1 mm3 segments and digested with 0.

Establishing a human pancreatic stem cell line and transplanting induced pancreatic islets to reverse experimental diabetes in rats.

The major obstacle in using pancreatic islet transplantation to cure type I and some type II diabetes is the shortage of the donors. One of ways to overcome such obstacle is to isolate and clone pancreatic stem cells as "seed cells" and induce their differentiation into functional islets as an abundant transplantation source. In this study, a monoclonal human pancreatic stem cell (mhPSC) line was obtained from abortive fetal pancreatic tissues.