Publications by authors named "Amy Sapkota"

Antibiotics are often used to treat severe infections, with third-generation cephalosporins and tetracyclines combined or fluoroquinolones alone being recommended by the US Centers for Disease Control and Prevention. Increases in antibiotic resistance of both environmental and clinical vibrios are of concern; however, limited longitudinal data have been generated among environmental isolates to inform how resistance patterns may be changing over time. Hence, we evaluated long-term trends in antibiotic resistance of vibrios isolated from Chesapeake Bay waters (Maryland) across two 3-year sampling periods (2009-2012 and 2019-2022).

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  • Tobacco use alters the oral microbiome, affecting the diversity and abundance of bacterial communities in smokers and smokeless tobacco users compared to non-users over a 4-month period.
  • Cigarette and smokeless tobacco users showed a higher diversity of bacteria, with more Firmicutes and fewer Proteobacteria, while non-users had more beneficial genera like Actinomyces and Neisseria.
  • Some bacterial species shifted significantly over time, and opportunistic pathogens were identified in tobacco users, providing new insights into the oral health impacts of different tobacco products.
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  • Alternative irrigation waters can contain harmful bacterial foodborne pathogens that may contaminate fruits and vegetables, making detection crucial for food safety.
  • A study compared qPCR (real-time PCR) with traditional culture methods for identifying these pathogens, examining factors like water type, season, and sample volume over two years across multiple sites.
  • The findings revealed that qPCR detection had reasonable predictive values, with better agreement observed in spring and summer, particularly in reclaimed and pond water compared to river water, indicating water type influences detection accuracy.
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  • - The study analyzed vibriosis trends in Maryland from 2006-2019, finding a significant 39% increase in incidence rates, particularly with V. vulnificus infections rising by 53%.
  • - From 2013-2019, hospitalizations due to vibriosis surged by 58%, with older adults and those from the upper eastern shore being the most affected.
  • - The findings highlight a long-term rise in Vibrio infections, emphasizing the need for better public awareness and environmental management strategies to mitigate future risks.
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Both SARS-CoV-2 and smoking tobacco adversely impact the respiratory system, damaging the airways and impairing lung function. While some studies have identified a positive association between smoking and increased susceptibility to COVID-19 infections, a few papers have concluded that smokers may be protected against such infections. Given these contradictory findings, there is an ongoing debate in the scientific community about whether or not smokers have a stronger predisposition towards COVID-19 infections.

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Enteric bacterial pathogen levels can influence the suitability of irrigation water sources for fruits and vegetables. We hypothesize that stable spatial patterns of Salmonella enterica and Listeria monocytogenes levels may exist across surface water sources in the Mid-Atlantic U.S.

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  • A study was conducted to analyze the bacterial communities in various tobacco products, including cigarettes, little cigars, cigarillos, and hookah, as well as a research cigarette product.
  • The researchers extracted DNA from 414 samples and sequenced a specific gene region to identify the bacteria present, focusing on metabolically-active (live) cells in a subset of 56 samples.
  • Key findings showed distinct microbiomes for each tobacco product, with specific bacteria linked to each type, including concerns about the presence of harmful pathogens in commercial products.
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  • Young adults are turning to hookahs as seemingly healthier smoking alternatives, but this exposes them to harmful microorganisms and carcinogenic compounds.
  • The study investigated bacterial communities in popular hookah brands at various storage conditions, revealing significant differences between brands and flavors over time.
  • Key findings highlight diverse bacterial taxa present in hookah tobacco, indicating potential health risks, and emphasize the need for further research on the transmission of these bacteria to users.
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  • The study analyzed the presence and ESBL activity of E. coli in surface and reclaimed water across the mid-Atlantic U.S. and found that phylogroups B1 and A were the most and least common, respectively.
  • Water type influenced the distribution of phylogroups, with group A being more prevalent in reclaimed water compared to other types.
  • Although general resistance to cephalosporins was low, specific groups exhibited higher resistance rates and the presence of resistance genes was linked to the water source, providing insights on water safety and public health.
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  • Climate change is impacting irrigation sources, leading to the need for evaluating alternative water sources like harvested rainwater for safe agricultural use.
  • A study in Frederick, Maryland, assessed the water, soil, and produce quality from vegetable rain gardens using rainwater irrigation, focusing on E. coli and other pathogens.
  • Results showed that the harvested rainwater and produce were within food safety guidelines, suggesting these systems can help address water challenges while ensuring food safety.
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Multiple studies have demonstrated that cigarettes harbor bacterial pathogens. Yet, to our knowledge, there are no published data to date on whether or not these microorganisms can be aerosolized and transmitted to the respiratory tract of users. To address this knowledge gap, we characterized cigarette bacterial communities and evaluated whether or not they could be aerosolized in mainstream smoke.

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  • The study aimed to assess the safety of irrigation water by examining antimicrobial resistance (AMR) in Enterococcus species, which could harbor AMR traits.
  • Over two years, researchers collected 333 water samples from various sites, identifying 198 unique Enterococcus isolates and discovering significant levels of single and multidrug resistance, especially in Enterococcus faecium found in reclaimed water.
  • Findings revealed a seasonal variation in AMR prevalence, indicating that understanding these patterns is crucial for regulating the safety of irrigation water for crops.
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Background: Increasing number of studies have linked air pollution exposure with renal function decline and disease. However, there is a lack of data on its impact among end-stage kidney disease (ESKD) patients and its potential modifying effect from extreme heat events (EHE).

Methods: Fresenius Kidney Care records from 28 selected northeastern US counties were used to pool daily all-cause mortality (ACM) and all-cause hospital admissions (ACHA) counts.

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  • The FSMA Produce Safety Rule mandates farmers to create a Microbial Water Quality Profile (MWQP) using samples from their agricultural water sources to monitor E. coli levels, which can be costly and time-consuming.
  • Research conducted on 279 water samples from various sites in the U.S. Mid-Atlantic aimed to evaluate whether testing frequency could be reduced without jeopardizing accuracy.
  • Results indicated that, under specific conditions, a smaller sub-sample (as few as 5 samples) could sufficiently demonstrate compliance with the safety standards, potentially easing the financial burden on farmers.
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Irrigation water sources have been shown to harbor foodborne pathogens and could contribute to the outbreak of foodborne illness related to consumption of contaminated produce. Determining the probability of and the degree to which these irrigation water sources contain these pathogens is paramount. The purpose of this study was to determine the prevalence of Salmonella enterica and Listeria monocytogenes in alternative irrigation water sources.

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Background: Typical thermoregulatory responses to elevated temperatures among healthy individuals include reduced blood pressure and perspiration. Individuals with end-stage kidney disease (ESKD) are susceptible to systemic fluctuations caused by ambient temperature changes that may increase morbidity and mortality. We investigated whether pre-dialysis systolic blood pressure (preSBP) and interdialytic weight gain (IDWG) can independently mediate the association between ambient temperature, all-cause hospital admissions (ACHA), and all-cause mortality (ACM).

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Background: Infections with nontyphoidal Salmonella cause an estimated 19,336 hospitalizations each year in the United States. Sources of infection can vary by state and include animal and plant-based foods, as well as environmental reservoirs. Several studies have recognized the importance of increased ambient temperature and precipitation in the spread and persistence of Salmonella in soil and food.

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Water scarcity has resulted in extensive wastewater recycling for agricultural irrigation in both Israel and the Palestinian Territories. However, minimal data have been collected regarding perceptions about wastewater recycling between the populations in these two areas. While geographically close and economically linked, these two populations differ in terms of governance, income, and access to technology for wastewater recycling.

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  • Previous studies focused on bacterial communities in commercial menthol and non-menthol cigarettes but lacked details on chemical constituents, which this study addresses using controlled SPECTRUM research cigarettes.
  • The research involved incubating cigarettes with different nicotine and menthol levels under specific conditions, then analyzing the resulting bacterial DNA to identify community changes.
  • Findings revealed that nicotine levels and menthol presence significantly influenced the abundance of certain bacteria, suggesting potential implications for respiratory health and the transfer of bacteria to users.
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The COVID-19 pandemic has had an enormous impact on education globally, forcing the teaching community to think outside the box and create innovative educational plans to benefit students at home. Here, we narrate how the undergraduate, laboratory-based Summer Internship Program of our CONSERVE Center of Excellence, which focuses heavily on engaging women and underrepresented minorities in STEM programming, took a turn from an in-person research experience to a fully virtual one. We share our challenges and how we overcame them.

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Background: Ponds are important freshwater habitats that support both human and environmental activities. However, relative to their larger counterparts (e.g.

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  • Enteric viruses (EVs) are a major cause of foodborne illnesses, and their persistence in the environment poses contamination risks for agricultural crops, especially through nontraditional irrigation sources like reclaimed and surface waters.
  • A 17-month study focused on detecting specific human EVs and indicators in Mid-Atlantic region irrigation waters, using real-time quantitative PCR to assess viral presence and environmental factors.
  • The results indicated higher detection rates of EVs and PMMoV in reclaimed water compared to surface water, showing significant correlations with water salinity and highlighting the need for microbiological analysis to ensure food safety.
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  • Tobacco is the main cause of preventable diseases and deaths in the U.S. and beyond, and tobacco manufacturers are required to report harmful chemicals in their products, but not microorganisms.
  • Over 50 years of research has found more than 89 types of bacteria and 19 types of fungi in various tobacco products, with some being known pathogens and others potential threats.
  • Future studies should investigate how viable these microorganisms are and whether they can transfer to users' respiratory systems, leading to health issues.
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