Hematology is a routine component of clinical management in veterinary patients. Anticoagulant choice can profoundly influence morphologic assessment of erythrocytes, leukocytes, thrombocytes, and their subsequent quantification. Previous chelonian studies suggest that lithium heparin (LH) is a superior anticoagulant due to hemolysis resulting from dipotassium ethylenediaminetetraacetic acid (dEDTA) in some species.
View Article and Find Full Text PDFAnticoagulants prevent clotting of blood samples and preserve cellular morphology for hematologic evaluations, but studies comparing anticoagulants are limited in snakes. The objective of this study was to evaluate the effects of lithium heparin (LH) and dipotassium ethylenediaminetetraacetic acid (EDTA) on hematologic values in prairie rattlesnakes (PR; , n = 16) and Lake Erie water snakes (LEWS; , n = 21). Venipuncture was performed and blood samples were immediately aliquoted into LH and EDTA microtainers.
View Article and Find Full Text PDFPreservation of blood through use of anticoagulants allows delayed assessment of hematologic health and is commonly employed in veterinary health assessments. The two most common anticoagulants are lithium heparin (LH) and dipotassium ethylenediaminetetraacetic acid (EDTA), and their effects can vary widely between species. The hematologic effects of these anticoagulants on blood from European starlings () have not been established, and these birds could serve as models for passerine species both in managed collections and in the wild.
View Article and Find Full Text PDFSouth American sea lions (Otaria byronia) and Peruvian fur seals (Arctocephalus australis) are sympatric species inhabiting the coastal Peruvian marine ecosystem. Declining abundance has prompted population health monitoring programs, including temporal monitoring of blood parameters. Several methods exist to determine total leukocyte count, yet no studies have evaluated agreement between methods in pinnipeds.
View Article and Find Full Text PDFBackground: Acute phase protein (APP) measurement is used to detect inflammation. Intramuscular (IM) injections could cause tissue injury and induce an acute phase response (APR).
Objectives: To evaluate the effects of IM procaine penicillin G (PPG) injections on APP concentrations in horses.
Hematology and biochemistry testing of boas and pythons is a valuable topic for practicing clinicians and researchers alike. This article reviews blood cell morphology (with accompanying images) and reviews the literature for hematologic and biochemical material clinically relevant to the families Boidae and Pythonidae.
View Article and Find Full Text PDFDetermination of the biological variation of laboratory tests is essential for accurate interpretation during health assessments. Few studies investigate the biological variation of CBC variables in reptiles, and none of these involve squamates. Thus, we investigated the biological variation of hematology parameters in bearded dragons () to determine if conventional population-based reference intervals are appropriate in evaluating single laboratory samples.
View Article and Find Full Text PDFBackground: The diagnosis of neoplastic cavitary effusions requires the identification of neoplastic cells in effusions, yet the cytologic appearance of neoplastic effusions can be highly variable due to the varied mechanisms of formation. Additional parameters might aid in the interpretation of equivocal cytologic results.
Objectives: Our goal was to evaluate whether total protein concentrations can be used to support the diagnosis of neoplasia in the peritoneal and pleural effusions of dogs with lower cellularities (≤5000 nucleated cells/μL).
In collaboration with the American College of Veterinary Pathologists.
View Article and Find Full Text PDFInvestigations focusing on host-ectoparasite interactions in animals have revealed asymptomatic to severe health and fitness consequences suggesting that species mobilize different interspecific response mechanisms. Fewer studies, however, have examined intraspecific responses to ectoparasitic burdens. In this study, we analyzed host health and fitness responses to increasing ectoparasite burdens along with the presence/absence of hemoparasites of free-ranging insular rock iguanas () in The Bahamas.
View Article and Find Full Text PDFBackground: Oxidative stress inhibits albumin's ability to complex with cobalt. Feline serum-cobalt binding has not been described.
Objectives: The objective was to develop a cobalt binding test for use with feline serum, and correlate the results with other biochemical and cellular constituents in blood, and with clinical diseases of cats.
Vet Clin North Am Small Anim Pract
January 2012
Neutropenia is a serious hematopathologic change that should not be ignored. In almost all patients, it is an important primary or secondary indicator of significant underlying disease. While in some neutropenic patients the diagnostic work up will be simple, in others it is challenging.
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