Syngeneic immune-dependent abortions in mice suggest paternal alloantigen-independent mechanisms.

Problem: Recurrent immune-associated miscarriages in humans are thought to result from maternal immune responses to paternal alloantigens. We investigated the role of paternal alloantigens in a mouse model of immune-dependent abortion.

Method Of Study: Sib-crosses of C57Bl/6J (haplotype b/b) mice heterozygous for a targeted hypomorphic allele of the tbp gene (tbp(deltaN/+)) resulted in selective mid-gestational abortion of 88% of the tbp(deltaN/deltaN) fetuses.

Molecular genotyping of the mouse scid allele.

Severe combined immunodeficiency (SCID) mice, which lack mature B- and T-cells, provide an important system for studies on immunity and disease. However, since the scid mutation is a single T-to-A transversion, genotypic analysis can be problematic. We have developed a rapid and simple sequence-based analysis that provides identification of both the scid and the wild-type (+) allele.