Prospective, double-blinded, randomised controlled trial assessing the effect of an Octenidine-based hydrogel on bacterial colonisation and epithelialization of skin graft wounds in burn patients.

Background: Moist wound treatment improves healing of skin graft donor site wounds. Microbial colonised wounds represent an increased risk of wound infection; while antimicrobially active, topical antiseptics may impair epithelialization.

Objectives: The aim of this prospective randomised controlled clinical trial was to examine the influence of an Octenidine-dihydrochloride (OCT) hydrogel on bacterial colonisation and epithelialization of skin graft donor sites.

[Humeral fracture due to arm wrestling - an indirect fracture in a doped athlete].

Background: Humeral shaft fractures due to recreational arm wrestling are reported from time to time.

Case Report: We report the case of a 19 year old, anabolic steroids up taking, recreational arm wrestler who sustained a spiral fracture of the right humeral bone and secondary radial nerve palsy. We discuss the surgical treatment and the biomechanics of this rare injury mechanism.

Investigations on prophylactic effects of coarse feed structure and/or potassium diformate on the microflora in the digestive tract of weaned piglets experimentally infected with Salmonella Derby.

The purpose of this study was to examine potential prophylactic effects of a coarse feed structure and/or potassium diformate (KDF as feed additive) addition to diets on the microflora of the digestive tract in reared piglets experimentally infected with Salmonella Derby. The results show that coarse grinding as well as KDF addition are able to influence positively the intestinal flora and are capable to reduce Salmonella excretion of infected piglets. Coarse grinding of main ingredients (e.

Comparison of methods for antimicrobial susceptibility testing and MIC values for pleuromutilin drugs for Brachyspira hyodysenteriae isolated in Germany.

In Germany treatment of swine dysentery is hampered by Brachyspira hyodysenteriae strains showing elevated MIC values to the few antibiotics licensed. Therefore, susceptibility testing of clinical isolates is an important service to the swine practitioner. This study compares the established agar dilution procedure for antimicrobial susceptibility testing of this fastidious anaerobe to the broth microdilution test newly developed [Anim.

Diagnostic multiplex PCR for toxin genotyping of Clostridium perfringens isolates.

In this study we provide a protocol for genotyping Clostridium perfringens with a new multiplex PCR. This PCR enables reliable and specific detection of the toxin genes cpa, cpb, etx, iap, cpe and cpb2 from heat lysed bacterial suspensions. The efficiency of the protocol was demonstrated by typing C.

[The prevalence of Actinobaculum suis in boars of breeding herds in the Omsk region (Russian Federation) by indirect immunofluorescence technique].

Actinobaculum suis (Corynebacterium suis, Eubacterium suis, Actinomyces suis) was detected in the preputial diverticulum of 64,8% of 162 boars investigated in 8 districts of the region Omsk (Russian Federation) by indirect immunofluorescent technique. Until yet no informations were available about the prevalence of Actinobaculum (A.) suis in swine herds of the Russian Federation.

[Chronic-idiopathic enteropathy in cats--a case control study].

Aim of this study is to clinically, microbiologically and histopathologically characterize inflammatory bowel disease (IBD) in the cat. Nine cats with chronic persistent or intermitent vomitus and diarrhea were examined between 1998 and 2001. All cats had a thuorough diagnostic workup performed.

Pathophysiological measurement and results after laparoscopic fundoplication for gastroesophageal reflux disease.

Purpose: Both surgical and conservative treatments for gastroesophageal reflux disorder (GERD) are controversial. The aim of this prosepective study was to examine outcomes after laparoscopic antireflux surgery.

Methods: The subjects were 143 patients who underwent laparoscopic antireflux surgery.

Laparoscopic-endoscopic rendezvous resection of gastric tumors.

Background: Submucosal and mucosal tumors of the stomach display a wide spectrum of histopathologic and prognostic characteristics. Biopsies obtained using endoscopic techniques often do not provide the representative histologic sample needed for further therapeutic decisions.

Methods: From 1999 to 2002, 18 patients with gastric tumors underwent a combined endoscopic-laparoscopic local resection of the tumors using two different procedures and were prospectively analyzed.

[Diagnosis of swine dysentery and spirochaetal diarrhea. III. Results of cultural and biochemical differentiation of intestinal Brachyspira species by routine culture from 1997 to 1999].

A survey is given on the occurrence and distribution of different Brachyspira species in pigs, in the northwest of Germany. In total 2975 specimen (feces, fecal swabs, colon) were taken and sent for laboratory analysis during the years 1997 to 1999. 1218 Brachyspira (B.

[Investigations on Brachyspira--diagnostic and therapeutic strategies in swine dysentery].

The infectious agent of swine dysentery, Brachyspira (Br.) hyodysenteriae, seems to be widespread in German pig herds. Due to different reasons the eradication is increasingly difficult.

[Diagnosis of swine dysentery and spirochaetal diarrhea. 2. Effort of macrorestriction analysis for differentiation of intestinal Serpulina relative to determination by culture-biochemical markers following species classification].

Genotypic differentiation by means of macrorestriction fragment profile analysis using Mlul restriction enzyme was carried out differentiating 41 Serpulina field strains from swine (38), dog (2) and a rat as well as ten type and reference strains into 40 electrophoretic types. A dendrogram was created using the average linkage between groups method. At a level of 50% similarity the patterns could be divided into six groups that roughly corresponded to the results yielded by cultural and biochemical methods formerly (FELTRUP et al.

[The diagnosis of swine dysentery and spirochaete diarrhea. 1. Cultural-biochemical differentiation of intestinal Serpulina in routine diagnosis].

Frequent incidence of Serpulina strains showing all cultural and biochemical characteristics of Serpulina (S.) hyodysenteriae except of being indole negative, and alpha-galactosidase positive isolates showing strong haemolysis on Columbia agar with 5% sheep blood and trypticase soy agar with 5% ox blood, respectively, was the cause to evaluate common biochemical and cultural methods in Serpulina routine diagnostics. To this purpose ten type and reference strains as well as 47 field strains were examined for their ability to produce indole, haemolysis, hippurate cleavage, alpha-galactosidase, alpha- and beta-glucosidase activity.

[Investigations into the occurrence and the antibiotic susceptibility of gram negative anaerobes of the genera Bacteroides, Prevotella, Porphyromonas and Fusobacterium in specimens obtained from diseased animals].

From different samples of 247 diseased animals (cattle, sheep, goat, horse, pig, dog, cat, rodent, zoo-animals), 410 strains of gram-negative anaerobes were cultured. 297 isolates (72.4%) could be differentiated to the species level by using cultural-biochemical methods, gaschromatography and cell-wall-lipidanalysis.

[Demonstration of pneumonia in swine as a constant problem: culture and immunofluorescence microscopic studies of bronchioalveolar lavage (BAL) and serological findings].

Bronchoalveolar lavage was performed in 128 pigs from five fattening units showing acute pneumonia (48 animals), subclinical purulent pneumonia (17 animals), and chronic purulent pneumonia (63 animals). These samples were investigated for bacteria. Additionally immunofluorescence microscopy as well as serological investigations were performed to detect antibodies against several bacteria and viruses.

[Serologic examinations for the detection of antibodies against Eubacterium suis in swine].

Determination of antibodies against Eubacterium suis (E. suis) in the serum of pigs was carried out by an indirect immunofluorescence test. Therefore 35 sows with urinary tract infections as well as 43 healthy control sows were investigated.

Characterization of Staphylococcus intermedius from healthy dogs and cases of superficial pyoderma by DNA restriction endonuclease patterns.

In order to study the possible clonal relation of Staphyloccocus (S.) intermedius from canine superficial pyoderma and from healthy carriers, isolates from pustular swabs and from vaginal, nasal and normal skin sabs were typed using macrorestriction analysis with Sma I and pulsed-field gel electrophoresis. From the size of the resulting fragments the size of the chromosome of S.

[The occurrence and significance of enterotoxin-producing Clostridium perfringens strains in the intestinal tract of horses].

100 faecal samples from clinically healthy horses of different age groups and feeding habits, 50 samples of faeces from horses suffering from enteropathy accompanied by diarrhoea and small and/or large intestine from 25 horses that had died after an intestinal disease were examined for the presence of Clostridium (Cl.) perfringens. The frequency with which Cl.

[The diagnosis of listeria encephalitis in ruminants using cultural and immunohistologic methods. II. Immunohistologic studies in formalin-fixed paraffin sections].

The unlabelled peroxidase-antiperoxidase (PAP)-technique was compared with the avidin-biotin-peroxidase-complex (ABC)-technique in the identification of Listeria antigen in formalin-fixed paraffin sections of 58 ruminant brains, 44 of which showed histopathological lesions typical for listeric encephalitis. Rabbit hyperimmune serum, obtained by immunization with a Listeria monocytogenes strain of serotype 1/2 a, served as primary serum in the immunohistochemical investigations. The antiserum was tested for specificity using formalin-fixed smears of various bacterial species.

[The diagnosis of Listeria encephalitis in ruminants using cultural and immunohistological techniques. I. Comparison of different selective media and culture techniques for the detection of Listeria from ruminant brains].

The selective L-PALCAMY differential enrichment broth, the Listeria enrichment broth of the International Dairy Federation, Oxford Listeria selective agar, and PALCAM Listeria selective agar were comparatively examined in the cultural isolation of Listeria spp. from ten ruminant brains. The L-PALCAMY medium proved to be superior to the IDF broth in both selectivity and productivity for Listeria spp.

[The effect of bacterial interference phenomena in experimental Staphylococcus hyicus infections of gnotobiotic piglets].

The prevention of exudative epidermitis could be confirmed in experimental investigations with gnotobiotic piglets when the skin first was colonized with avirulent strains of Staphylococcus (Staph.) hyicus and subsequently exposed to virulent strains of Staph. hyicus.

[Bacteriologic studies of the occurrence of Chlamydia psittaci in organs of swine and in aborted swine fetuses].

By inoculation of embryonating chicken eggs via the yolk sac route Chlamydia psittaci was grown from 11 lungs of 45 pigs with pneumonia (24.4%). From the lungs of 55 pigs with other diseases the organism was isolated in five cases (9.

[The occurrence of enterotoxigenic Clostridium perfringens strains in the feces of dogs and cats].

A total of 147 faecal specimen of dogs and cats was examined with cultural method for the occurrence of Cl. perfringens and its enterotoxin by using a reversed passive latex agglutination test (Pet-RPLA). Cl.