Aqueous-based Binary Sulfide Nanoparticle Inks for CuZnSnS Thin Films Stabilized with Tin(IV) Chalcogenide Complexes.

CuZnSnS(CZTS) is a promising semiconductor material for photovoltaic applications,with excellent optical and electronic properties while boasting a nontoxic, inexpensive, andabundant elemental composition. Previous high-quality CZTS thin films often required eithervacuum-based deposition processes or the use of organic ligands/solvents for ink formulation,which are associated with various issues regarding performance or economic feasibility. To addressthese issues, an alternative method for depositing CZTS thin films using an aqueous-basednanoparticle suspension is demonstrated in this work.

Heterologous expression of lipase in Escherichia coli is limited by folding and disulfide bond formation.

Functional expression of lipase B from Pseudozyma antarctica (PalB) in the cytoplasm of Escherichia coli BL21(DE3) and its mutant derivative Origami B(DE3) was explored. Coexpression of DsbA was found to be effective in enhancing PalB expression. The improvement was particularly pronounced with Origami B(DE3) as a host, suggesting that both folding and disulfide bond formation may be major factors limiting PalB expression.