Comparative evaluation of three herbal extracts on growth performance, immune response, and resistance against Vibrio parahaemolyticus in Litopenaeus vannamei.

This study evaluated the effects of dietary supplementation with herbal extracts from Artemisia herba-alba, Lonicera japonica, and Lilium candidum on growth performance, survival, feed utilization, antioxidant capacity, and immune response in Litopenaeus vannamei. The efficacy of these herbal-supplemented diets was assessed in enhancing resistance against Vibrio parahaemolyticus-induced Acute Hepatopancreatic Necrosis Disease (Vp ). A total of 2,400 shrimp post-larvae (initial weight 0.

Prevalence and molecular characterization of Acute hepatopancreatic necrosis disease (AHPND) in cultured white-leg shrimp Litopenaeus vannamei with the fungal bioactive control strategy.

Mass mortalities with clinical signs characteristic of acute hepatopancreatic necrosis disease (AHPND) were reported in cultured Litopenaeus vannamei from three Egyptian farms: Wadi-Mariote, Berket Ghalyoun, and Qarun Lake. During 4-month surveillance in 2023, shrimp samples were collected to investigate the prevalence of AHPND-causing Vibrio parahaemolyticus (Vp) based upon phenotypic identification, PCR, and DNA sequencing of pirA genes and pirB toxin genes followed by maximum likelihood phylogenetic analysis. In addition, the pathogenicity of V.

Development of silver nano-based indirect ELISA and Dot-ELISA methods for serological diagnosis of a bacterial fish pathogen Aeromonas veronii.

Rapid and accurate detection of bacterial pathogens is critical in controlling disease outbreaks affecting farmed fish. The present study aimed to develop a novel serological diagnostic approach using nano‑silver based Enzyme-linked immunosorbent assay (ELISA) for speedy detection of Aeromonas veronii infections in Nile tilapia. A.

Livoneca redmanii (Isopoda, Cymothoidae) in meagre Argyrosomus regius: parasitological and molecular diagnosis and proposed control measure.

Isopodiosis in cultured meagre Argyrosomus regius was investigated at 3 farms in the northern lakes of Egypt throughout 2018, based upon prevalence rate, parasitological examination, and molecular identification by PCR targeting the large ribosomal subunit 16S of the rRNA gene. Further, the susceptibility of A. regius to isopod infection was experimentally evaluated under hyposalination of 25, 15, and 8 ppt for 1 wk.

Amyloodiniosis in cultured Dicentrarchus labrax: parasitological and molecular diagnosis, and an improved treatment protocol.

Amyloodinium ocellatum, the causative agent of amyloodiniosis (marine velvet, velvet disease), affects marine and brackish fish in various warm and temperate habitats. We recorded disease outbreaks with high morbidity and mortality rates in marine-cultured European seabass Dicentrarchus labrax fry at 2 locations in northwest Egypt. The sudden outbreak, high morbidity and mortality rates, and skin lesions with a velvety appearance in affected fish all indicated A.

Epizootics of Pseudomonas anguilliseptica among cultured seabream (Sparus aurata) populations: Control and treatment strategies.

The present study was investigating the clinical pictures, prevalence, as well as the ecological conditions associated with Pseudomonas anguilliseptica outbreaks in four cultured seabream, Sparus aurata farms at different localities in Egypt during winter of 2016. The phenotypic and genotypic patterns of Pseudomonas isolates were investigated. The existence of intraspecific heterogeneity among different isolates was analyzed using Restriction Fragment Length Polymorphism (RFLP) technique.