Publications by authors named "Amol B Tayade"
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- The high altitude trans-Himalayan region is challenging for survival, yet the algae there, specifically Spirogyra porticalis, have evolved to produce unique molecules that help them adapt to harsh climatic conditions.
- The study analyzed this alga using various scientific methods and identified 19 amino acids, 9 fatty acids, and 8 vitamins, with alanine, proline, and lysine being the most prevalent amino acids.
- The findings suggest that S. porticalis could be a valuable nutraceutical supplement due to its potential benefits in combating oxidative stress.
Article Synopsis
- * A total of nine essential and twelve non-essential amino acids were identified, with histidine, lysine, and threonine being the most abundant among the essential ones.
- * The lipid content showed a high presence of saturated fatty acids and several important unsaturated fatty acids, alongside significant levels of essential dietary minerals like calcium, potassium, and iron.
Article Synopsis
- In India, Rhipicephalus (Boophilus) microplus ticks have developed resistance to traditional acaricides, prompting research into alternative solutions.
- The study evaluated the effectiveness of Ageratum conyzoides extracts, finding that the ethanolic extract could significantly reduce egg-laying and was effective against various resistant tick populations.
- The research identified key bioactive compounds within the plant, suggesting its potential for developing a new plant-based acaricide to manage tick infestations in cattle and buffaloes.
The freshwater alga Spirogyra porticalis (Muell.) Cleve, a filamentous charophyte, collected from the Indian trans-Himalayan cold desert, was identified on the basis of morpho-anatomical characters. Extracts of this alga were made using solvents of varying polarity viz.
View Article and Find Full Text PDFFourteen saxicolous lichens from trans-Himalayan Ladakh region were identified by morpho-anatomical and chemical characteristics. The n-hexane, methanol and water extracts of the lichens were evaluated for their antioxidant capacities. The lichen extracts showing high antioxidant capacities and rich phenolic content were further investigated to determine their cytotoxic activity on human HepG2 and RKO carcinoma cell lines.
View Article and Find Full Text PDFWe estimated the nutritive value, vitamin content, amino acid composition, fatty acid content, and mineral profile of a phytococktail comprising sea buckthorn (Hippophae rhamnoides), apricot (Prunus armeniaca), and roseroot (Rhodiola imbricata) from trans-Himalaya. The free vitamin forms in the phytococktail were determined by rapid resolution liquid chromatography/tandem mass spectrometry (RRLC-MS/MS). Vitamin E and B-complex vitamins were detected as the principle vitamins.
View Article and Find Full Text PDFBackground: Himalayan plants are widely used in traditional system of medicine both as prophylactics and therapeutics for high altitude maladies. Our aim was to evaluate the antioxidant capacities and bioactive compounds of methanol and n-hexane extracts of the phytococktail comprising of sea buckthorn (Hippophae rhamnoides), apricot (Prunus armeniaca) and roseroot (Rhodiola imbricata) from trans-Himalaya.
Methods: The 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and nitric oxide (NO) radical scavenging capacities and lipid peroxidation inhibition (LPI) property of the extracts were determined.
A rapid method was developed to determine both types of vitamins in Rhodiola imbricata root for the accurate quantification of free vitamin forms. Rapid resolution liquid chromatography/tandem mass spectrometry (RRLC-MS/MS) with electrospray ionization (ESI) source operating in multiple reactions monitoring (MRM) mode was optimized for the sequential analysis of nine water-soluble vitamins (B1, B2, two B3 vitamins, B5, B6, B7, B9, and B12) and six fat-soluble vitamins (A, E, D2, D3, K1, and K2). Both types of vitamins were separated by ion-suppression reversed-phase liquid chromatography with gradient elution within 30 min and detected in positive ion mode.
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- Rhodiola imbricata, known as Rose root or Arctic root, is a significant food crop and medicinal herb found in the Indian trans-Himalayan cold desert, belonging to the Crassulaceae family.
- A chemometric analysis of its root extracts using gas chromatography mass spectrometry (GC/MS) identified 63 phyto-chemotypes, including various alcohols, fatty acids, and terpenoids, highlighting its complex chemical composition.
- The biological activities of these identified compounds suggest they have a range of positive pharmacological effects, indicating potential for future research on isolating these phyto-chemotypes for medicinal use.