Aptamer-Based Gold Nanoparticles-PDMS Composite Stamps as a Platform for Micro-Contact Printing.

In the present study, a functional template made up of in situ synthesised gold nanoparticles (AuNPs) is prepared on polydimethylsiloxane (PDMS) for patterning of target protein onto the desired solid substrates. Unlike previous studies in which bioreceptor probes are randomly attached to the PDMS stamp through electrostatic interactions, herein, we propose an AuNPs-PDMS stamp, which provides a surface for the attachment of thiol-modified biorecognition probes to link to the stamp surface through a dative bond with a single anchoring point based on thiol chemistry. By using this platform, we have developed the ability for microcontact printing (µCP) to selectively capture and transfer target protein onto solid surfaces for detection purposes.

Ag Functionalized InO Derived From MIL-68(In) as an Efficient Electrochemical Glucose Sensor.

In this study, Ag@InO modified nickel foam (NF) was reported for its role as a non-enzymatic glucose sensor. Ag@InO was prepared by a simple two-step method; preparation of a metal-organic framework (MOF) MIL-68(In) by solvothermal method, entrapment of Ag + by adding AgNO then drying it for 2 h to complete the entrapment process and subsequent calcination at 650°C for 3 h. The Ag@InO modified NF was employed as a non-enzymatic glucose sensor to determine glucose concentrations in an alkaline medium.

Aptamer Laden Liquid Crystals Biosensing Platform for the Detection of HIV-1 Glycoprotein-120.

We report a label-free and simple approach for the detection of glycoprotein-120 (gp-120) using an aptamer-based liquid crystals (LCs) biosensing platform. The LCs are supported on the surface of a modified glass slide with a suitable amount of B40t77 aptamer, allowing the LCs to be homeotropically aligned. A pronounced topological change was observed on the surface due to a specific interaction between B40t77 and gp-120, which led to the disruption of the homeotropic alignment of LCs.

Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein.

Surface protein gp-120 of HIV-1 virus plays an important role in the infection of HIV-1, but detection of gp-120 during the early stage of infection is very difficult. Herein, we report a binding bioassay based on an RNA aptamer B40t77, which binds specifically to gp-120. The bioassay is built upon a hydrophobic glass slide with surface immobilized gp-120.

Liquid crystals based sensing platform-technological aspects.

In bulk phase, liquid crystalline molecules are organized due to non-covalent interactions and due to delicate nature of the present forces; this organization can easily be disrupted by any small external stimuli. This delicate nature of force balance in liquid crystals organization forms the basis of Liquid-crystals based sensing scheme which has been exploited by many researchers for the optical visualization and sensing of many biological interactions as well as detection of number of analytes. In this review, we present not only an overview of the state of the art in liquid crystals based sensing scheme but also highlight its limitations.