Deciphering the Potential of Probiotics in Vaccines.

The demand for vaccines, particularly those prepared from non-conventional sources, is rising due to the emergence of drug resistance around the globe. Probiotic-based vaccines are a wise example of such vaccines which represent new horizons in the field of vaccinology in providing an enhanced and diversified immune response. The justification for incorporating probiotics into vaccines lies in the fact that that they hold the capacity to regulate immune function directly or indirectly by influencing the gastrointestinal microbiota and related pathways.

Multifunctional integration of tungsten oxide (WO) coating: A versatile approach for enhanced performance of antibiotics against single mixed bacterial infections.

Nanomaterials containing tungsten (TNMs), characterized by diverse nanostructures had been extensively used in biomedical sector. Despite numerous reports focusing on TNM applications in specific biomedical areas, there is a noticeable absence of comprehensive studies that focused on detailed characterization of nanomaterials along with their biological applications. The present work described the structural, morphological, and antimicrobial properties of tungsten oxide (WO) nanoparticles coated by antibiotics (nanobiotics), and their application on single and mixed bacterial culture.

Resistance Modulation of Individual and Polymicrobial Culture of and through Nanoparticle-Coupled Antibiotics.

Polymicrobial mastitis is now becoming very common in dairy animals, resulting in exaggerated resistance to multiple antibiotics. The current study was executed to find drug responses in individual and mixed Culture of and isolated from milk samples, as well as to evaluate the antibacterial potential of tungsten oxide nanoparticles. These isolates (alone and in mixed culture) were further processed for their responses to antibiotics using the disc diffusion method.

Validating Interactions of Pathogenic Proteins of and with Phytochemicals of and .

This study focused on the assessment of the antimicrobial resistance of () and () isolated from bovine mastitis milk samples and the revealing anti-mastitis potential of phytocompounds of and through molecular docking analysis. The mastitis milk samples were collected from various dairy farms for the isolation of the bacteria ( and ) and their response to antibiotics. Ethanolic extracts of both plants were prepared.

Sodium Alginate-Based MgO Nanoparticles Coupled Antibiotics as Safe and Effective Antimicrobial Candidates against of Houbara Bustard Birds.

Alternative and modified therapeutic approaches are key elements in culminating antibiotic resistance. To this end, an experimental trial was conducted to determine the cytotoxicity and antibacterial potential of composites of magnesium oxide (MgO) nanoparticles and antibiotics stabilized in sodium alginate gel against multi-drug-resistant isolated from a houbara bustard. The characterization of preparations was carried out using X-ray diffraction (XRD), scanning transmissible electron microscopy (STEM), and Fourier-transform infrared spectroscopy (FTIR).

Modification of the drug resistance of emerging milk-borne pathogens through sodium alginate-based antibiotics and nanoparticles.

and are emerging as major milk-borne pathogens. Additionally, resistance to antibiotics of pathogens is of concern. Therefore, this study investigated the prevalence and drug resistance of and in mastitis milk samples and assessed the antimicrobial potential of sodium alginate (G)-stabilized MgO nanoparticles (M) and antibiotics (tylosin [T] and ampicillin [A]) against both of these pathogens.

Recent Global Trends in Vaccinology, Advances and Challenges.

Vaccination/immunization plays a key role in maintaining the optimum health of humans and animals where the prevalence of disease is high and treatment becomes less effective [...

MRSA compendium of epidemiology, transmission, pathophysiology, treatment, and prevention within one health framework.

is recognized as commensal as well as opportunistic pathogen of humans and animals. Methicillin resistant strain of (MRSA) has emerged as a major pathogen in hospitals, community and veterinary settings that compromises the public health and livestock production. MRSA basically emerged from MSSA after acquiring SCC element through gene transfer containing responsible for encoding PBP-2α.

Influence of baking and frying conditions on acrylamide formation in various prepared bakery, snack, and fried products.

The core objective of the present study was to evaluate the influence of baking/frying times and temperatures on the formation of acrylamide in bakery, snack, and fried products such as biscuits, muffins, pizza, cakes, samosa, paratha rolls, nuggets, and potato cutlets during baking/frying at different times and temperature conditions. First of all, the raw material, especially flour, was tested for its proximate composition and rheological characteristics. The quantification of acrylamide produced during the processing of different products was carried out through the HPLC method.

Role of Bacteriophages for Optimized Health and Production of Poultry.

The poultry sector is facing infections from , , and ., and , that have developed multidrug resistance aptitude. Antibiotics cause disturbances in the balance of normal microbiota leading to dysbiosis, immunosuppression, and the development of secondary infections.

A new insight in immunomodulatory impact of botanicals in treating avian coccidiosis.

Avian coccidiosis is caused by genus i.e. and and lead to three billion US dollar per year economic loss in poultry industry and reduces the growth performance of birds.

Enhancing activity of β-lactam and fluoroquinolones antibiotics by artemisinin and its derivatives against MDR .

Artemisinin and its derivatives had played a biocidal role in biomedical remedies, while they were expected to enhance the activity of antibiotics against multiple drug-resistant (MDR) bacteria. The current study evaluated the interaction of artemisinin (ART), dihydroartemisinin (DHA), artesunate (AS), and artemisinic acid (AA) with β-lactam and fluoroquinolones antibiotics against . Antibiotic strip test (-test), Kirby Bauer's disc test (KB method), and broth microdilution method were adopted for susceptibility analysis, while the checkerboard method was applied to assess synergisms.

Vancomycin drug resistance, an emerging threat to animal and public health.

The need to supply quality food for the growing human population has led to the revolutionization of food production and processing in recent years. Meanwhile, food production sources are at risk of microbial attack, while the use of antibiotics to counter them is posing another threat to food safety and security. Vancomycin was used as the first line of defense against multiple drug-resistant bacteria salient of which is methicillin-resistant .

Effects of different composting methods on antibiotic-resistant bacteria, antibiotic resistance genes, and microbial diversity in dairy cattle manures.

Composting is a common practice used for treating animal manures before they are used as organic fertilizers for crop production. Whether composting can effectively reduce microbial pathogens and antibiotic resistance genes remain poorly understood. In this study, we compared 3 different dairy manure composting methods-anaerobic fermentation (AF), static compost (SC), and organic fertilizer production (OFP)-for their effects on antibiotic-resistant bacteria, antibiotic resistance genes, and microbial community diversity in the treated manures.

Correction: Shabbir et al. Effect of Yeast-Fermented Citrus Pulp as a Protein Source on Nutrient Intake, Digestibility, Nitrogen Balance and In Situ Digestion Kinetics in Nili Ravi Buffalo Bulls. 2021, , 1713.

Awais Shabbir was mistakenly written as "Muhammad Awais" in the original publication [...

Evaluation of Cytotoxic Potential of Extracts on Human Hepatocarcinoma HepG2 and Normal HEK293T Cell Lines.

Data regarding the therapeutic potential of () are insufficient. It becomes more important to explore plants as an alternative or palliative therapeutics in deadly diseases around the globe. The current study was planned to explore for its anticancer activity of ethanolic and hexane extracts of leaves against hepatic carcinoma (HepG2) and human embryonic kidney (HEK293T) cell lines.

Genomic and Therapeutic Analyses of Isolated from Cattle Reproductive Tract.

is emerging as a ubiquitous multidrug-resistant pathogen circulating among animals, humans, and their environment. The current study focused on molecular epidemiology and evidence-based treatment against from bovine endometritis. For this study, = 304 cattle were screened for endometritis using ultrasonography while presenting case history, and clinical signs were also considered.

Evaluation of Sodium Alginate Stabilized Nanoparticles and Antibiotics against Drug Resistant Isolated from Gut of Houbara Bustard Bird.

Alternative approaches and/or modified approaches to tackle resistance in gut microbes are need of the hour. The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against () isolated from the gut of Houbara bustard bird ( = 105 fecal samples). The preparations consisted of gel stabilized ampicillin (G+A), gel stabilized MgO and ampicillin (G+M+A), gel stabilized MgO and cefoxitin (G+M+C), gel stabilized tylosin (G+T), gel stabilized MgO and tylosin (G+M+T), and gel stabilized MgO (M+G).

Acaricidal Potential and Ecotoxicity of Metallic Nano-Pesticides Used against the Major Life Stages of Ticks.

Ticks (Acari: Ixodidae) are blood-feeding parasites capable of transmitting diseases to animals (Piroplasmosis) and humans (Congo fever, Lyme disease). The non-judicious use of chemical acaricides has led to the development of acaricide-resistant ticks, making the control of ticks and tick-borne diseases difficult. This study reports the efficacy of magnesium oxide (MgO), iron oxide (FeO), and zinc oxide (ZnO) nanoparticles (NPs) as alternatives to traditional acaricides/pesticides using in vitro tests against major representative stages of ticks.

Divergent Analyses of Genetic Relatedness and Evidence-Based Assessment of Therapeutics of from Semi-intensive Dairy Systems.

Use of antibiotics without following standard guidelines is routine practice in developing countries which is giving rise to genetic divergence and increased drug resistance. The current study analyzed genetic divergence and drug resistance by and therapeutic efficacy of novel antibiotic combinations. The study revealed that 42.

Tracking Infection and Genetic Divergence of Methicillin-Resistant at Pets, Pet Owners, and Environment Interface.

() has become a leading animal and public health pathogen that keeps on transferring from one host to other, giving rise to newer strains by genetic shifts. The current study was designed to investigate the epidemiology and genetic relatedness of gene in isolated from pets, immediate individuals in contact with pets, and veterinary clinic environments. A total of = 300 samples were collected from different veterinary hospitals in Pakistan using convenience sampling.

Insights into Nanopesticides for Ticks: The Superbugs of Livestock.

Livestock is an integral part of agriculture countries where ticks play significant role as potent pests causing considerable losses to economy and health. Drug resistance has made these pests supersede conventional therapies and control programs Nanotechnology here comes as an advancing and significant candidate alternatively able to reverse drug resistance. Nanoparticles, hence, against ticks may better be considered as nanopesticides that act in ways other than conventional drug efficacies.