Transcriptome analysis of spermatogenically regressed, recrudescent and active phase testis of seasonally breeding wall lizards Hemidactylus flaviviridis.

Background: Reptiles are phylogenically important group of organisms as mammals have evolved from them. Wall lizard testis exhibits clearly distinct morphology during various phases of a reproductive cycle making them an interesting model to study regulation of spermatogenesis. Studies on reptile spermatogenesis are negligible hence this study will prove to be an important resource.

Role of estrogen in the regulation of spermatogenesis in the Indian wall lizard Hemidactylus flaviviridis.

The role of estrogen in the Indian wall lizard, Hemidactylus flaviviridis during PMSG induced spermatogenesis in the regression phase and during normal spermatogenesis in the active breeding phase was investigated. Blood hormone levels demonstrated a high testosterone to estrogen ratio in the breeding and vice verse during the regressed phase. PMSG treatment (30 IU in 100 μl saline/lizard/alternate day for 30 days) during the regressed phase stimulated spermatogenesis which was associated with a significant (p<0.