Publications by authors named "Amit Singh Sahrawat"
Stereoselective synthesis of quaternary stereocenters represents a significant challenge in organic chemistry. Herein, we describe the use of ene-reductases OPR3 and YqjM for the efficient asymmetric synthesis of chiral 4,4-disubstituted 2-cyclohexenones via desymmetrizing hydrogenation of prochiral 4,4-disubstituted 2,5-cyclohexadienones. This transformation breaks the symmetry of the cyclohexadienone substrates, generating valuable quaternary stereocenters with high enantioselectivities (ee, up to >99%).
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- * Current treatment for CD is difficult due to a lack of effective and safe drugs, prompting research into the enzyme TcAkt as a potential drug target.
- * By using advanced techniques like Nuclear Magnetic Resonance and Molecular Dynamics simulations, the study identifies how TcAkt interacts with specific ligands and proposes a mechanism for its activation, showing that existing human inhibitors can potentially target TcAkt for drug development.
The reduction of C=X (X = N, O) bonds is a cornerstone in both synthetic organic chemistry and biocatalysis. Conventional reduction mechanisms usually involve a hydride ion targeting the less electronegative carbon atom. In a departure from this paradigm, our investigation into Old Yellow Enzymes (OYEs) reveals a mechanism involving transfer of hydride to the formally more electronegative nitrogen atom within a C=N bond.
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