Purpose: Emergence of multidrug resistance in Neisseria gonorrhoeae, an STI of public health significance is the biggest challenge to gonorrhoea control. Monitoring for antimicrobial resistance is essential for the early detection of emergent drug resistance patterns.
Methodology: One hundred and twenty four N.
Objectives: Validation of a cost effective in-house method for HIV-1 drug resistance genotyping using plasma samples.
Design: The validation includes the establishment of analytical performance characteristics such as accuracy, reproducibility, precision and sensitivity.
Methods: The accuracy was assessed by comparing 26 paired Virological Quality Assessment (VQA) proficiency testing panel sequences generated by in-house and ViroSeq Genotyping System 2.