Nanozyme-strip based on MnO nanosheets as a catalytic label for multi-scale detection of aflatoxin B with an ultrabroad working range.

Lateral flow immunoassay (LFIA) is the most effective real-time detection method for aflatoxin B (AFB). Here, we constructed a nanozyme-strip based on MnO nanosheets (MnO NSs) as a catalytic label for detection of AFB. By taking advantage of the MnO-TMB catalytic amplified system, the new test achieves rapid detection with high sensitivity and ultrawide range.

A direct competitive nanozyme-linked immunosorbent assay based on MnO nanosheets as a catalytic label for the determination of fumonisin B.

A direct competitive nanozyme-linked immunosorbent assay (dcNLISA) based on MnO nanosheets (MnO NSs) as a nanozyme label was developed for the highly sensitive determination of fumonisin B (FB). MnO NS-labeled fumonisin B-bovine serum albumin was easily synthesized as a competing antigen for the dcNLISA. And color changes derived from the MnO-3,3',5,5'-tetramethylbenzidine (TMB) system were exploited as the output signals of the dcNLISA.