Publications by authors named "Amina Sayed"
Article Synopsis
- STAT3-hyper IgE syndrome (STAT3-HIES) is a genetic immune disorder causing lung issues and other symptoms.
- CRISPR-Cas9 technology has been utilized to correct a specific mutation (p.R382W) associated with this condition in patient-derived fibroblasts and iPSCs, achieving about 29%-30% correction efficiency without harmful off-target effects.
- The treated cells demonstrated improved STAT3 signaling and maintained their ability to develop into functional alveolar organoids, suggesting ABE-based gene editing could be a promising treatment for STAT3-HIES.
Overexpression of p53 has been found in many types of human malignancy. The present study aimed to detect preoperative serum p53 among 158 patients with different gastrointestinal cancers using ELISA technique based on mouse anti-p53 DO-7 monoclonal antibody and anti-p53 rabbit polyclonal antibody. A single band of 53kDa was detected in nuclear protein tissue extracts of selected cancer patients and in 96% of the corresponding sera using Western blot assay.
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