Evaluation of Neutrophil Activation Biomarkers in Response to Programmed Cell Death Protein-1 (PD-1) and Toll-like Receptor 9 (TLR-9) Inhibition in Triple Negative Breast Cancer.

Background And Objective: Activation of neutrophils has proven to be useful in different models of cancer therapy. However, more comprehensive studies are required to further characterize these potential targets. Thus, we aimed to evaluate the effects of programmed cell death protein-1 (PD-1) and toll-like receptor 9 (TLR-9) inhibition on markers of neutrophil activation in different breast cancer subtypes.

Foxi2 and Sox3 are master regulators controlling ectoderm germ layer specification.

In vertebrates, germ layer specification represents a critical transition where pluripotent cells acquire lineage-specific identities. We identify the maternal transcription factors Foxi2 and Sox3 to be pivotal master regulators of ectodermal germ layer specification in . Ectopic co-expression of Foxi2 and Sox3 in prospective endodermal tissue induces the expression of ectodermal markers while suppressing mesendodermal markers.

Effect of laser wavelength on soil carbon measurements using laser-induced breakdown spectroscopy.

We investigate the effect of laser wavelength on laser-induced breakdown spectroscopy (LIBS) on the measurement of carbon in agricultural soils. Two laser wavelengths, 1064 nm and 532 nm, were used to determine soil carbon concentration. No chemical pretreatment, grinding, or pelletization was performed on soil samples to simulate in-field conditions.

The association of human cytomegalovirus with biomarkers of inflammation and immune activation in breast cancer.

Background: The presence of human cytomegalovirus (HCMV) in breast cancer has been reported, suggesting a potential association between HCMV infection and breast carcinogenesis.

Objective: To evaluate the association between HCMV infection and immune activation and inflammatory markers in breast cancer.

Methods: HCMV DNA was detected from all patients using real-time PCR, Anti HCMV IgM and IgG antibodies were measured.

The immunoregulatory axis (programmed death-1/programmed death ligand-1) on CD4+ T cells in lupus nephritis: association with vitamin D and chemokine C-X-C motif ligand 12.

Lupus nephritis (LN) is one of the most serious complications of systemic lupus erythematosus (SLE). Multiple immunomodulatory mechanisms contribute to the pathogenesis of LN. A deep understanding of the immunopathogenesis of LN is essential to identify optimal molecular targets, as most immunotherapeutic algorithms are still based on unselective drugs.

Profiling NSD3-dependent neural crest gene expression reveals known and novel candidate regulatory factors.

The lysine methyltransferase NSD3 is required for the expression of key neural crest transcription factors and the migration of neural crest cells. Nevertheless, a complete view of the genes dependent upon NSD3 for expression and the developmental processes impacted by NSD3 in the neural crest was lacking. We used RNA sequencing (RNA-seq) to profile transcripts differentially expressed after NSD3 knockdown in chick premigratory neural crest cells, identifying 674 genes.

Modulation of IL-4 level by fludarabine and its relation to apoptosis in chronic B-cell lymphocytic leukemia.

Unlabelled: Accumulation of malignant B-lymphocytes in chronic B-cell lymphocytic leukemia (B-CLL) is mainly attributed to reduced apoptosis rather than increased proliferation rate. Interleukin-4 (IL-4) has been proved to be involved in the survival mechanisms of B-cells as well as protection of B-CLL cells against spontaneous or drug induced apoptosis. Fludarabine is one of purine analogs and the current standard treatment for B-CLL, which has been proved to induce apoptosis in normal and malignant lymphocytes.

The role of interferon-gamma and vitamin-A in modulating telomerase activity in breast cancer patients.

Telomerase is a ribonucloprotein enzyme that appears to play a role in carcinogenesis. To understand the regulatory mechanisms which govern telomerase activity, we used telomeric repeat amplification protocol (TRAP) to quantitate and compare telomerase activity in cultured peripheral blood mononuclear cells (PBMCs) with and without INF-gamma in breast cancer patients. Serum levels of IFN-gamma and vitamin-A were also measured to look for a possible influence of these chemotherapeutic agents on telomerase activity.