Publications by authors named "Amin Shahedi"

Article Synopsis
  • * Out of 373 samples, 27% displayed piroplasm infections, predominantly from Theileria spp. (78 samples) and Babesia spp. (23 samples), with a molecular detection rate of 38%.
  • * The main findings indicated that Theileria ovis was prevalent, while Babesia ovis was the primary cause of Babesiosis, highlighting the importance of molecular diagnostics for accurate identification, especially in co-infection scenarios.
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Background: The production of bovine theileriosis vaccine involves in vitro cultivation of schizont-infected cell lines. Fetal bovine serum (FBS) is commonly used in animal cell culture, including the cell line. However, we aimed to reduce the amount of serum needed for cell culture by modifying the Stoker culture medium with supplements such as excretion factor and serum substitutes.

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The present study aimed at evaluating the presence of tick-borne apicomplexan parasites including Theileria ovis, Theileria lestoquardi, Theileria annulata, and Theileria orientalis in 92 cattle and 105 sheep from 6 different districts of Guilan and Mazandaran Provinces, in the southern littoral of Caspian Sea. Furthermore, ixodid ticks were collected from the same animals. Stained blood smears were microscopically evaluated for the presence of blood parasites, and a specific PCR was applied for the detection of Theileria species.

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