Mapping Cell Viability Quantitatively and Independently From Cell Density in 3D Gels Noninvasively.

Objective: In biomanufacturing there is a need for quantitative methods to map cell viability and density inside 3D bioreactors to assess health and proliferation over time. Recently, noninvasive MRI readouts of cell density have been achieved. However, the ratio of live to dead cells was not varied.