Human dental pulp mesenchymal stem cells isolation and osteoblast differentiation.

Aim: This study was focused on the isolation and characterization of mesenchymal stem cells (MSCs) from human dental pulp (DPSC).

Methods: The study was performed in the Department for Oral and Cranio-Maxillo- Facial Surgey Hamad Medical Corporation, Doha, Qatar and Weill Cornell Medical Colleague Doha, Qatar, in period 2010-2011. Dental pulp was extracted from premolars and third molars of 19 healthy patients.

Rat bone marrow stem cells isolation and culture as a bone formative experimental system.

Bone marrow mesenchymal cells have been identified as a source of pluripotent stem cells with multipotential potential and differentiation in to the different cells types such as are osteoblast, chondroblast, adipoblast. In this research we describe pioneering experiment of tissue engineering in Bosnia and Herzegovina, of the isolation and differentiation rat bone marrow stromal cells in to the osteoblast cells lineages. Rat bone marrow stromal cells were isolated by method described by Maniatopulos using their plastic adherence capatibility.

In vivo osteoinductive effect and in vitro isolation and cultivation bone marrow mesenchymal stem cells.

Bone marrow contains cell type termed mesenchymal stem cells (MSC), first recognized in bone marrow by a German pathologist, Julius Cohnheim in 1867. That MSCs have potential to differentiate in vitro in to the various cells lines as osteoblast, chondroblast, myoblast and adipoblast cells lines. Aims of our study were to show in vivo capacity of bone marrow MSC to produce bone in surgically created non critical size mandible defects New Zeland Rabbits, and then in second part of study to isolate in vitro MSC from bone marrow, as potential cell transplantation model in bone regeneration.

Pharmacokinetics and biological effect of the recombinant human bone morphogenetic protein-7.

Background: During Bone Morphogenetic Protein (BMP) induced osteoblastogenesis and bone formation, strong vascularization is observed at the transition of preosteoblasts to mature osteoblasts. The cellular and molecular mechanisms that determine autonomous hematopoietic competence in the BMP induced osteoblastogenesis and angiogenesis have not been characterized yet.

Aims: In the present study we investigated, whether rhBMP-7 in collagen as carrier (ACS) stimulates osteoblastogenesis have effect on the physiologic autonomous hematopoetic system and clinical condition on animal model.

Recombinant human bone morphogenetic protein-7 and bone marrow as a substitute for bone graft in reconstruction defect of rabbit mandible.

Objective: To obtain information on the feasibility of employing recombinant human bone morphogenetic protein-7 (rhBMP-7), mixed with bone marrow as a substitute for autologous bone graft.

Methods: We carried out the study in the University Clinic Center, Sarajevo, Bosnia and Herzegovina, from February 2002 to January 2004. Six New Zealand rabbits underwent hemiresection of the mandible.

[Biological activities of bone morphological protein in bone regeneration].

Bone matrix contents various development factors which control structuring and absorption and those factors play important role in bone and cartilage development. Bone morphological proteins are members of TGF-beta super family and their activity is certain becoming from the bone. This activity leads to the serial development processes which include chemo taxis, proliferation and differentiation which results in trans resistant formation of cartilage as well as production of life cells of a bone tissue.

Clinical and anatomy study of the human mental foramen.

The great diffusion of the surgical techniques in jaws surgery and the progress of the radiological imagining procedures expressed many interest in clinical anatomy of the mental foramen (MF). The study goal was to determine the precise location of the MF and the surrounding anatomical landmarks. Measurements of the MF position relative to the surgical landmarks and related posterior teeth were made on 20 dry mandibles with complete dentition and intact alveolar bridge obtained from the Institute of Anatomy, School of Medicine, University in Sarajevo.

[Use of new computer technologies in surgical education--virtual surgery].

Education is a process of accepting knowledge and skills or manners modification throught training. One of the most important characteristics in surgery education are classical education program through adequate literature and observation in operation room. Lip and palate clefts are relative rare diseases and classical knowledge acquirement of surgery methods in this field is sometimes defficient.