Transcriptomic profiling of ' Liberibacter asiaticus' in different citrus tissues reveals novel insights into Huanglongbing pathogenesis.

' Liberibacter asiaticus' (Las) is a gram-negative bacterial pathogen associated with citrus huanglongbing (HLB) or greening disease. Las is transmitted by the Asian citrus psyllid (ACP) where it colonizes the phloem tissue, resulting in substantial economic losses to citrus industry worldwide. Despite extensive efforts, effective management strategies against HLB remain elusive, necessitating a deeper understanding of the pathogen's biology.

Playing with FIRE: How an RXLR Oomycete Effector Fuels Disease by Hijacking 14-3-3 Proteins.

The plant pathogen causes rot disease in several monocots and dicots. The plant 14-3-3 proteins are targets of different types of effector molecules secreted by the pathogens. An RXLR-type effector FIRE (14-3-3 interacting RXLR effector) and its target 14-3-3 proteins that localize to haustoria have been identified, pointing to a potential site of interaction.

Effector Identification in Plant Pathogens.

Effectors play a central role in determining the outcome of plant-pathogen interactions. As key virulence proteins, effectors are collectively indispensable for disease development. By understanding the virulence mechanisms of effectors, fundamental knowledge of microbial pathogenesis and disease resistance have been revealed.

RpoS contributes in a host-dependent manner to colonization of the leaf apoplast during plant disease.

Contaminated fresh produce has been routinely linked to outbreaks of Salmonellosis. Multiple studies have identified factors associated with successful colonization of diverse plant niches and tissues. It has also been well documented that can benefit from the conditions generated during plant disease by host-compatible plant pathogens.

How do bacteria transform plants into their oasis?

Plant pathogenic bacteria rely on aquatic and nutritive microenvironments to proliferate within the host. In this issue of Cell Host & Microbe, Hu et al., Roussin-Léveillée et al.

Transcriptome Profiling of ' Liberibacter asiaticus' in Citrus and Psyllids.

' Liberibacter asiaticus' (Las) is an emergent bacterial pathogen that is associated with the devastating citrus huanglongbing (HLB). Vectored by the Asian citrus psyllid, Las colonizes the phloem tissue of citrus, causing severe damage to infected trees. So far, cultivating pure Las culture in axenic media has not been successful, and dual-transcriptome analyses aiming to profile gene expression in both Las and its hosts have a low coverage of the Las genome because of the low abundance of bacterial RNA in total RNA extracts from infected tissues.

Pattern-Triggered Immunity Alters the Transcriptional Regulation of Virulence-Associated Genes and Induces the Sulfur Starvation Response in Pseudomonas syringae pv. tomato DC3000.

Pattern-triggered immunity (PTI) can confer broad defense against diverse microbes and pathogens with disparate lifestyles through the detection of microbial extracellular signatures by surface-exposed pattern recognition receptors. However, unlike recognition of pathogen effectors by cytosolic resistance proteins, PTI is typically not associated with a host-cell programmed cell death response. Although host PTI signaling has been extensively studied, the mechanisms by which it restricts microbial colonization are poorly understood.

Validation of RT-qPCR Approaches to Monitor Pseudomonas syringae Gene Expression During Infection and Exposure to Pattern-Triggered Immunity.

Pseudomonas syringae pv. tomato DC3000 is an important model plant pathogen, with a fully annotated genome and multiple compatible plant hosts. Very few studies have examined the regulation of DC3000 gene expression in vivo.