Electrochemical immunoassay for lactalbumin based on the use of ferrocene-modified gold nanoparticles and lysozyme-modified magnetic beads.

The authors describe a method for electrochemical determination of the breast cancer biomarker α-lactalbumin (α-LA) using disposable screen-printed carbon electrodes (SPCEs). Lysozyme-conjugated FeO nanoparticles (Lys-FeONPs) were used to capture α-LA on the surface of the SPCEs which then is trapped in an immunosandwich using secondary antibodies labeled with ferrocene-modified gold nanoparticles. The amperometric response of ferrocene (recorded at +0.

Family-based association study in Tunisian familial psoriasis.

Background: The pathogenesis of all forms of psoriasis remains obscure. Segregation analysis and twin studies together with ethnic differences in disease frequency all point to an underlying genetic susceptibility to psoriasis, which is both complex and likely to reflect the action of a number of genes.

Materials And Methods: In the present study, we performed a family-based association study, and a transmission dysequilibrium test using the PLINK program, in a set of seven Tunisian multiplex families using a panel of 96 single-nucleotide polymorphisms localized in several regions across the genome.