Polarized microscope assisted study of collagen fibres in Oral Submucous Fibrosis and their clinicopathologic correlation: An in-vitro study.

Background: The present study was aimed to evaluate and analyze orientation and birefringence pattern of collagen fibers histopathologically in different grades of OSMF using the special stain (Picrosirius red) under polarizing microscope and relate these factors with the severity of OSMF.

Materials And Method: 45 confirmed cases of OSMF were taken from the archives. The selection of clinical cases was done according to classification by J.