Optimizing reproductive performance in pangasius catfish broodstock: A review of dietary and molecular strategies.

catfish, a significant player in the global whitefish market, encounters challenges in aquaculture production sustainability. Quality broodstock maintenance and seed production are impeded by growth, maturation, and fecundity issues. This review investigates the efficacy of strategic nutrient composition and molecular strategies in enhancing broodstock conditions and reproductive performance across various fish species.

Development of sperm cryopreservation protocol for patin buah, Pangasius nasutus.

The development of sperm cryopreservation for Pangasius nasutus is necessary in order to serve the growing demand of this species through artificial fertilization and the preservation of valuable strains of male broodstocks. In the present study, the basic protocol of sperm cryopreservation for P. nasutus was established by identifying the optimal conditions for optimum cryoprotectant, toxicity of cryoprotectants, extenders, freezing condition and dilution ratio.

Enhancing Reproductive Performance of Freshwater Finfish Species through Dietary Lipids.

Dietary lipid manipulation in the feed of commercially cultured finfish is used not only to improve production and culture but also to enhance their reproductive performances. The inclusion of lipid in broodstock diet positively affects growth, immunological responses, gonadogenesis, and larval survival. In this review, existing literature on the importance of freshwater finfish species to aquaculture and the inclusion of dietary lipid compounds in freshwater fish feed to accelerate the reproduction rate is being summarized and discussed.

Microalgal Preference and Feeding Density of Selected Microalgae Diets by Blue Swimming Crab (Linnaeus, 1758).

<b>Background and Objectives:</b> Blue swimming crab, <i>Portunus pelagicus</i> (Linnaeus, 1758) are economically important sources of soft-shell products for export demand. However, increasing over-exploitation and pollution towards the sea resources affected the production of the <i>P. pelagicus</i>.

Dietary Implications of Detoxified Kernel for Fingerlings.

Antinutritional components must be substantially reduced to ensure better utilization of unconventional feeds in animal nutrition. Among the different methods of processing, soaking represents a simple and inexpensive alternative. This study attempted to determine the nutritional properties of socked kernel (JCK) and the effect of its dietary inclusions on ( = 50; mean initial weight = 6.

A Review of the Nursery Culture of Mud Crabs, Genus : Current Progress and Future Directions.

The nursery stages of mud crab, genus , proceed from the megalopa stage to crablet instar stages. We review the definition and several of the key stages in mud crab nursery activities. The practice of the direct stocking of megalopa into ponds is not recommended due to their sensitivity.

Genetic Variation between Triploid and Diploid (Burchell, 1822) Using RAPD Markers.

This study was designed to examine the use of RAPD markers in discriminating triploid and diploid African catfish (Burchell, 1822). Following a routine technique, triploidy was induced by cold shock and confirm by erythrocyte measurement in . Thereafter, 80 RAPD markers were screened; out of which, three showed the highest percentage of polymorphism (i.

Performance Characteristics of in Different Culture Media and Concentration.

This study determined the effect of growth media and culture concentration on the growth, proximate, and microelement composition of . The culture of was done using three media, namely Modified COMBO Medium (COMBO), Bold's Basal Medium (BBM), and Bristol, at two concentrations (50% and 100%). The results obtained show that the cell density (>3.

Dietary implications of toasted Jatropha curcas kernel on the growth, haematology, and organ histology of Clarias gariepinus fingerlings.

The nutritional value of Jatropha curcas kernel (JCK) can be improved through different processing methods; however, when using a thermal treatment, optimization of the process is needed to prevent denaturation of nutrients. In this study, JCK was toasted for varying durations (0, 10, 20, and 30 min) and nutritionally evaluated. The implication of feeding Clarias gariepinus with dietary inclusions (35% CP; 315 kcal g) of the toasted JCK was also reported.

Optimization of ultraviolet irradiation of (Burchell, 1822) eggs for androgen production.

The optimum distance and duration of ultraviolet (UV) irradiation for the complete inactivation of African catfish Clarias gariepinus egg nucleus was investigated in this study. The UV light was suspended above the unfertilized eggs at four distances (5, 10, 20 and 30 cm) and for five durations (1, 2, 3, 4 and 5 min). Then, the irradiated eggs were activated with sperm from diploid C.

Sexual dimorphism of Malaysian Mahseer, broodstock reared in captivity: Morphometric measurements dataset.

Despite having high economical value and declining wild population, the aquaculture of the Malaysian mahseer () is still way behind. Crucial information such as on its sexual dimorphism is scarce thus making its broodstock management in captivity challenging. The first step in studying fish sexual dimorphism is by observing and identifying the morphological differences between each sex.

Hydrothermal Processing of (Burchell, 1822) Filets: Insights on the Nutritive Value and Organoleptic Parameters.

This study evaluated the effects of cooking for different hydrothermal-treatment durations (10, 20, 30 and 40 min) on the proximate composition, amino acid profile, fatty acid composition and organoleptic parameters of filets of African catfish (Burchell, 1822). Filets of the fish were prepared from market size African catfish with similar breeding history. Parameters of the processed filet under the different hydrothermal durations were also compared against a raw-unprocessed control group except during organoleptic analysis.

Fatty Acid Composition of Hepatopancreas and Gonads in Both Sexes of Orange Mud Crab, Cultured at Various Water Flow Velocities.

Nutritional quality of the hepatopancreas and gonads of orange portunid mud crab, was evaluated for each gender under four treatment of different water velocities (0, 20, 40 and 60 cm s), in terms of nutrient reserve and nutrient for reproduction. About 56 crabs were used in this study in which fatty acids composition was analysed using gas chromatography mass-spectrometry (GC-MS). For hepatopancreas analysis, monounsaturated fatty acids (MUFAs) were present in the highest fatty acids concentration, followed by polyunsaturated fatty acids (PUFAs) and, saturated fatty acids (SFAs).

Dataset for the morphological and erythrocytes parameters of and their reciprocal hybrids.

Discrimination of different fishes can be done through different means which includes morphological appearance. When two fishes are successfully hybridized, they produce progenies that have shared morphology between their pure parent, hence, making morphometric characterization an important aspect of hybrid discrimination. However, erythrocyte characterization is also a simpler method for characterization.

Microbiota composition data for wild and captive bluestreak cleaner wrasse (Valenciennes, 1839).

The is known as the "doctor fish" because of its role in removing parasites and infectious pathogens from the body of other fishes. This important role played both in wild and captive conditions could represent a novel form of parasitic transmission process mediated by the cleaning activity of the fish. Yet, there is a paucity of data on the microflora associated with this fish which is important for tracking disease infection and generally monitoring the health status of the fish.

Data on PCR primer design for glucose 6-phosphate dehydrogenase gene and the effects of dietary carbohydrate levels on its expression in the liver of Malaysian mahseer ().

The enzyme glucose-6-phosphate dehydrogenase (G6PD) catalyses the metabolite glucose-6-phosphate in producing NADPH during the first phase of pentose-phosphate pathway thus provides reducing power to all cells for cellular growth, antioxidant defence, and biosynthetic reactions in all living organism. The deliberate inclusion of starch as carbohydrate source in commercial feed however may affect the G6PD hepatic activity in cultured fish. We designed a set of primers to target G6PD gene in the popular Malaysian aquaculture species, For this dataset, the molecular characteristics of obtained G6PD () nucleotide sequence was analysed using multiple alignments and phylogenetic analyses of the deduced amino acids.

Aquaponics production of catfish and pumpkin: Comparison with conventional production systems.

Aquaponics is known to be a smart way of producing fish and crops simultaneously; however, there is a paucity of information about the extents of this system's efficiency over other conventional methods of food production. Thus, this study was designed to evaluate the performance of a catfish-pumpkin aquaponics system in comparison with recirculatory and static aquaculture systems (for fish performance), as well as irrigated and nonirrigated systems (for pumpkin performance). Results obtained showed that the production of fish in the aquaponics system was 29% and 75% more efficient than recirculatory and static aquaculture systems, respectively.

Experimental data of phospholipid supplementary in full fishmeal replacement diets on growth indices, whole body nutrient composition, muscle fatty acid composition, haematocrit value and serum lysozyme activity in Malaysian mahseer ().

The data collection was initiated to evaluate the effects of supplementary phospholipid to non-fishmeal based diet in order to make functional diets for the Malaysian Mahseer, . Four iso-nitrogenous and iso-lipidic diets were formulated to consist 100% fishmeal (FM100), 0% fishmeal or full fishmeal replacement (FM0), and 0% fishmeal supplemented with 4% phospholipids (FM0+4%PL), 6% phospholipids (FM0+6%PL). A 60-day feeding trial was conducted and data collection was carried out for the following parameters; growth indices, somatic parameters, whole body nutrient composition, muscle fatty acid composition, haematocrit value and serum lysozyme activity.

Experimental data on glucose and energy levels of orange mud crab, at different water velocities.

The present datasets were conducted to investigate glucose concentration in hemolymph, energy levels at selected body parts (hepatopancreas, muscle, gonad), and feces among different sexes of crabs cultured at four different water velocities (0, 20, 40, and 60 cm/s) during a 60-day culture period. A total of 102 immature crabs (51 males, and 51 females) were sampled from Kuala Muda, Kedah coastal water, Peninsular Malaysia (5°39'N 100°19'E) from April to November of 2018. Results indicated that glucose concentration was the highest at water velocity of 60 cm/s for both male and female crabs (♂: 3.

First Report on Successful Triploidy Induction in Clarias gariepinus (Burchell, 1822) Using Electroporation.

This study investigated the use of electric-shock in inducing triploidy in African catfish Clarias gariepinus. To achieve this, three voltages (9, 12, 21 V) were applied for different durations (3, 5, 10 min). The shock was initiated approximately three minutes after fertilization followed by incubation in ambient temperature.

Data on population density, growth, survival, water quality, larval stage index and ingestion rate of selected microalgae of portunids crabs at different feeding regimes.

Population density, growth, survival, water quality and larval stage index of purple mud crab, at different feeding regimes and the data on ingestion rate of chosen microalgae, survival and larval development of blue swimming crab, are presented. A twenty days of larval culture from zoeal 1 until megalopa stage under two different feeding regimes of A) Rotifer, nauplii and shrimp meat and B) Rotifer, nauplii and artificial feed is shared. A method on investigation of individual larvae of capability to catch four different types of microalgae within 24 h is also shared.

Experimental data on behavioral, hepatosomatic, gonadosomatic indeces and total lipid of mud crab, Scylla olivacea at different velocity levels.

The data collected in the present work correspond to the behavioral, Hepatosomatic Index (HSI), Gonadosomatic Index (GSI) and total lipid analysis between male and female mud crabs, at different water velocities. A total of 56 immature male and female crabs were used in this data article. The important criteria for estimating the selective habitat facing by is a considerate of (1) the behavioral range in response to abiotic factors (and how it adapt ontogenetically) and (2) the movement of the crab under wild velocities situations.

Data on the molting duration and time of hardening of instar crab at different culture temperatures.

This data article includes raw and analyze data for molting duration and time of hardening of blue swimming crab, instar at three different temperatures of 24 °C, 28 °C, and 32 °C. Two sets of experimental data are included: first, the recorded on time-lapse video of duration of successful molted crabs (from emergence of swimming legs to chelae); and second, the time interval of shell hardening up to before molting occurred. Shell hardening were calculated in 1 h interval with a unit of hour molting crab, meanwhile the molting duration of the crabs were calculated in second molting crab.

Molecular Characterization and Gene Expression of Glutathione Peroxidase 1 in Exposed to Temperature Stress.

Temperature is an abiotic factor that affects various biological and physiological processes in fish. Temperature stress is known to increase the production of reactive oxygen species (ROS) that subsequently cause oxidative stress. Fish is known to evolve a system of antioxidant enzymes to reduce ROS toxicology.