Publications by authors named "Amber Hann"
Article Synopsis
- Imbalances in proteolytic activity are linked to inflammatory bowel diseases (IBD), where intestinal proteases can disrupt homeostasis and promote inflammation through protease-activated receptors (PARs).
- This study focuses on the role of microbial proteases in activating PAR2 and found that proteolytic cleavage of PAR2 increases intestinal permeability and inflammation during colitis.
- Mice with a mutated, protease-resistant version of PAR2 showed less severe colitis, suggesting that targeting PAR2 cleavage by bacterial proteases could be a potential therapeutic approach for IBD.
Background & Aims: Intestinal epithelial cell (IEC) damage is a hallmark of celiac disease (CeD); however, its role in gluten-dependent T-cell activation is unknown. We investigated IEC-gluten-T-cell interactions in organoid monolayers expressing human major histocompatibility complex class II (HLA-DQ2.5), which facilitates gluten antigen recognition by CD4 T cells in CeD.
View Article and Find Full Text PDFEmerging evidence implicates microbial proteolytic activity in ulcerative colitis (UC), but whether it also plays a role in Crohn's disease (CD) remains unclear. We investigated the effects of colonizing adult and neonatal germ-free C57BL/6 mice with CD microbiota, selected based on high (CD-HPA) or low fecal proteolytic activity (CD-LPA), or microbiota from healthy controls with LPA (HC-LPA) or HPA (HC-HPA). We then investigated colitogenic mechanisms in gnotobiotic C57BL/6, and in mice with impaired Nucleotide-binding Oligomerization Domain-2 (NOD2) and Protease-Activated Receptor 2 (PAR2) cleavage resistant mice (; R38E-PAR2 respectively).
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