Isolation and antibacterial activity of anomanol B and other secondary metabolites from the stem bark of (Annonaceae).

A new Lanosta-7,9(11),22-trien-3,15,20-triol named Anomanol B together with five known compounds: manniindole , arborinine , polycarpol 8,9-dimethoxyphenanthridin-6(5)-one and 3-O--D-glucopyranosyl--sitosterol were isolated from the stem bark extract of by routine chromatography techniques. 8,9-dimethoxyphenanthridin-6(5)-one was reported from natural origin for the first time. The structures of the compounds were established by comprehensive elucidation of spectroscopic data and by comparison with literature data.

Inhibitory effects of 190 compounds against SARS-CoV-2 M protein: Molecular docking interactions.

COVID-19 has caused many deaths since the first outbreak in 2019. The burden on healthcare systems around the world has been reduced by the success of vaccines. However, population adherence and the occurrence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants are still challenging tasks to be affronted.

The Antiplasmodial Potential of Medicinal Plants Used in the Cameroonian Pharmacopoeia: An Updated Systematic Review and Meta-Analysis.

Malaria is a real public health problem. It is the leading cause of morbidity and mortality in the world. Research in herbal medicine has so far shown that the use of plants against malaria is not to be neglected.

Chemical constituents of two Cameroonian medicinal plants: L. and Burm. f. (Malvaceae) and their antiplasmodial activity.

An extensive phytochemical investigation of the EtOH/HO (7:3) extracts of L. and Burm. f.

A phenanthridin-6()-one derivative and a lanostane-type triterpene with antibacterial properties from (Oliv). Engl. & Diels (Annonaceae).

The chemical investigation of root extract by column chromatography techniques led to the isolation of eight compounds among which two previously unreported compounds; a lanostane-type triterpene, lanosta-7,9(11),23-triene-3β,15α-diol and an alkaloid, 9-hydroxy-8-methoxyphenanthridin-6()-one along with six known compounds: lanosta-7,9(11),24-triene-3β,21-diol , oxoanolobine , 3, 4-dihydroxybenzoic acid stigmasterol βsitosterol and 3-O-β-D-glucopyranosyl-β-stigmasterol Their structures were established from spectral data, mainly HR-ESIMS, 1 D and 2 D NMR and by comparison with literature data. The crude root and stem bark extracts (AMR and AMB) and the isolated compounds () were tested against nine Gram-negative bacteria using rapid -iodonitrotetrazolium chloride ≥97% (INT) microdilution technique. It was found that AMR, AMB and compound were active against the nine tested bacteria with MIC values ranging from 64 to 1024 µg/mL.

Epigarcinol and isogarcinol isolated from the root of Garcinia ovalifolia induce apoptosis of human promyelocytic leukemia (HL-60 cells).

Background: Plants from garcinia genus have been used for centuries against several diseases.

Objective: This study aimed to investigate the mechanism of apoptosis induced by epigarcinol and isogarcinol isolated from the root of Garcinia ovalifolia (Clusiaceae) on human promyelocytic leukemia (HL-60 cells).

Methods: Epigarcinol and isogarcinol were isolated from the root of G.

Induction of mitochondrial dependent apoptosis and cell cycle arrest in human promyelocytic leukemia HL-60 cells by an extract from Dorstenia psilurus: a spice from Cameroon.

Background: The use of edible plants is an integral part of dietary behavior in the West region of Cameroon. Dorstenia psilurus (Moraceae) is widely used as spice and as medicinal plant for the treatment of several diseases in Cameroon. The aim of this study is to investigate the cytotoxic and apoptotic potential of methanol extract of D.

Antimicrobial activities of the methanol extract, fractions and compounds from Ficus polita Vahl. (Moraceae).

Background: Many plants of the family Moraceae are used in the treatment of infectious diseases. Ficus polita Vahl., an edible plant belonging to this family is used traditionally in case of dyspepsia, infectious diseases, abdominal pains and diarrhea.

Antimicrobial activity of the crude extracts and five flavonoids from the twigs of Dorstenia barteri (Moraceae).

The aim of this study was to evaluate the antimicrobial activity of the crude extract of the twigs of Dorstenia barteri (DBT) as well as that of four of the five flavonoids isolated from this extract. Gram-positive bacteria (six species), Gram-negative bacteria (12 species) and fungi (four species) were used. The agar disc diffusion test was used to determine the sensitivity of the tested samples while the well micro-dilution was used to determine the minimal inhibition concentrations (MIC) and the minimal microbicidal concentration (MMC) of the active samples.

Prenylated flavonoids, monoterpenoid furanocoumarins and other constituents from the twigs of Dorstenia elliptica (Moraceae).

A monoprenylated flavan and two monoterpenoid substituted furanocoumarins were isolated from the twigs of Dorstenia elliptica along with 3-(3,3-dimethylallyl)-4,2',4'-trihydroxylchalcone, psoralen, bergapten, O-[3-(2,2-dimethyl-3-oxo-2H-furan-5-yl)butyl]bergaptol, beta-sitosterol and its beta-D-glucopyranoside. The structure of the flavan was determined as 6(1,1-dimethylallyl)-7,4'-dihydroxylflavan and the monoterpenoid substituted furanocoumarins were assigned as O-[3-(2,2-dimethyl-3-oxo-2H-furan-5-yl)-3-hydroxybutyl]-bergaptol and O-[2-(5-hydroxy-2,6,6-trimethyl-3-oxo-2H-pyran-2-yl)ethyl]bergaptol, respectively, using spectroscopic analysis, especially, 2D NMR spectra.

Effect of a mass treatment with praziquantel on the excretion in urine of a polysaccharide antigen used for diagnosis of schistosomiasis due to S. mansoni in Cameroon.

The detection in urine, with a monoclonal antibody, of an excreted polysaccharide antigen characteristic of the genus Schistosoma, allows evaluation of the effect of praziquantel used for mass treatment, in a focus of S. mansoni infection. Inhibition of the passive haemagglutination test, which was used for detecting the polysaccharide antigen in urine, is more sensitive for measuring prevalence than the determination of eggs in stools by means of direct examination and the formalin-ether concentration technique.

Detection with a monoclonal antibody of an antigen, characteristic of the genus Schistosoma, excreted in the urine.

In the host, the antigen excreted by schistosomes in the circulating blood is concentrated in the urine. A mouse monoclonal antibody of the IgM class type lambda, directed against an epitope of the intestinal epithelium of the adult worm, is obtained. The antigen found in the urine of the host as well as the monoclonal antibody has been previously characterized.