Automatic local resolution-based sharpening of cryo-EM maps.

Motivation: Recent technological advances and computational developments have allowed the reconstruction of Cryo-Electron Microscopy (cryo-EM) maps at near-atomic resolution. On a typical workflow and once the cryo-EM map has been calculated, a sharpening process is usually performed to enhance map visualization, a step that has proven very important in the key task of structural modeling. However, sharpening approaches, in general, neglects the local quality of the map, which is clearly suboptimal.

Validation of electron microscopy initial models via small angle X-ray scattering curves.

Motivation: Cryo electron microscopy (EM) is currently one of the main tools to reveal the structural information of biological macromolecules. The re-construction of three-dimensional (3D) maps is typically carried out following an iterative process that requires an initial estimation of the 3D map to be refined in subsequent steps. Therefore, its determination is key in the quality of the final results, and there are cases in which it is still an open issue in single particle analysis (SPA).

The Primary Obesity Surgery Endolumenal (POSE) procedure: one-year patient weight loss and safety outcomes.

Background: Obesity is at epidemic proportions and increasing. Bariatric surgical procedures have demonstrated better durable weight loss than diet and exercise; however, risk may limit adoption of these procedures. Endoscopic procedures may offer less risk, lower cost, and satisfactory results, but limited safety and outcome data is available.

Endoscopic sleeve gastroplasty for the treatment of obesity.

Background And Study Aims: Emerging endoscopic techniques are minimally invasive and can mimic the anatomic alterations achieved by surgical sleeve gastrectomy. The objective of this study was to evaluate endoscopic sleeve gastroplasty.

Patients And Methods: This was a prospective, single-center study of 20 patients who underwent flexible endoscopic suturing for endoluminal gastric volume reduction.