Publications by authors named "Amarijt S Sarpal"
Article Synopsis
- Algae biomass characterization is complex due to its intricate metabolites, making analytical methods crucial for understanding it.
- Two methods were utilized: DI-ESI-TWIM-MS for direct analysis without separation and UHPLC-HDMS for high-throughput screening, identifying 1251 compounds across 16 microalgae samples.
- The findings enhance the ability to classify algae based on metabolite profiles, aiding in the optimization of strains and conditions for biotechnological applications, particularly in biofuels production.
Direct methods based on (1)H NMR spectroscopic techniques have been developed for the determination of neutral lipids (triglycerides and free fatty acids) and polar lipids (glyceroglycolipids/phospholipids) in the solvent extracts of oleaginous microalgal biomasses cultivated on a laboratory scale with two species in different media. The chemical shift assignments observed in the (1)H and (13)C NMR spectra corresponding to unsaturated (C18:N, N = 1-3, C20:3, C20:5, C22:6, epoxy) and saturated (C14-C18) fatty acid ester components in a complex matrix involving overlapped resonances have been unambiguously confirmed by the application of 2D NMR spectroscopy (total correlation spectroscopy and heteronuclear single quantum coherence-total correlation spectroscopy). The study of the effect of a polar lipid matrix on the determination of neutral lipids by an internal reference blending process by a systematic designed experimental protocol has provided absolute quantification.
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