Pre-existing heterosubtypic immunity provides a barrier to airborne transmission of influenza viruses.

Human-to-human transmission of influenza viruses is a serious public health threat, yet the precise role of immunity from previous infections on the susceptibility to airborne infection is still unknown. Using the ferret model, we examined the roles of exposure duration and heterosubtypic immunity on influenza transmission. We demonstrate that a 48 hour exposure is sufficient for efficient transmission of H1N1 and H3N2 viruses.

Quantitative live cell imaging reveals influenza virus manipulation of Rab11A transport through reduced dynein association.

Assembly of infectious influenza A viruses (IAV) is a complex process involving transport from the nucleus to the plasma membrane. Rab11A-containing recycling endosomes have been identified as a platform for intracellular transport of viral RNA (vRNA). Here, using high spatiotemporal resolution light-sheet microscopy (~1.

Non-Uniform and Non-Random Binding of Nucleoprotein to Influenza A and B Viral RNA.

The genomes of influenza A and B viruses have eight, single-stranded RNA segments that exist in the form of a viral ribonucleoprotein complex in association with nucleoprotein (NP) and an RNA-dependent RNA polymerase complex. We previously used high-throughput RNA sequencing coupled with crosslinking immunoprecipitation (HITS-CLIP) to examine where NP binds to the viral RNA (vRNA) and demonstrated for two H1N1 strains that NP binds vRNA in a non-uniform, non-random manner. In this study, we expand on those initial observations and describe the NP-vRNA binding profile for a seasonal H3N2 and influenza B virus.

Live Imaging of Influenza Viral Ribonucleoproteins Using Light-Sheet Microscopy.

Influenza viruses exhibit a complex life cycle that is still poorly understood. It involves independent replication of each of the eight segments that make up its genome and subsequent coordinated assembly as they egress from the host cell. Fast, time-resolved volumetric live cell imaging offers a powerful tool for understanding the various host mechanisms hijacked by the virus.

Intracellular Colocalization of Influenza Viral RNA and Rab11A Is Dependent upon Microtubule Filaments.

Influenza A virus (IAV) consists of eight viral RNA (vRNA) segments that are replicated in the host cell nucleus and transported to the plasma membrane for packaging into progeny virions. We have previously proposed a model where subcomplexes of vRNA are exported from the nucleus and assembled en route to the plasma membrane. However, the role of host cytoskeletal proteins in the cytoplasmic assembly of IAV vRNA segments remains unknown.

Pulse-shaping based two-photon FRET stoichiometry.

Förster Resonance Energy Transfer (FRET) based measurements that calculate the stoichiometry of intermolecular interactions in living cells have recently been demonstrated, where the technique utilizes selective one-photon excitation of donor and acceptor fluorophores to isolate the pure FRET signal. Here, we present work towards extending this FRET stoichiometry method to employ two-photon excitation using a pulse-shaping methodology. In pulse-shaping, frequency-dependent phases are applied to a broadband femtosecond laser pulse to tailor the two-photon excitation conditions to preferentially excite donor and acceptor fluorophores.

Fundamental eigenmode of traveling-wave phase-sensitive optical parametric amplifier: experimental generation and verification.

We investigate experimentally the eigenmodes of a Gaussian-beam-pumped traveling-wave phase-sensitive optical parametric amplifier (PSA). By varying the waist of an input LG(00) signal mode, we show that PSA performance improves with increasing spatial overlap between the input and the theoretically predicted fundamental eigenmode. For optimum waist, we report amplification and deamplification markedly higher than those observed for the traditional case of signal waist=√2× (pump waist).

All-optical modulation of four-wave mixing in an Rb-filled photonic bandgap fiber.

We demonstrate efficient all-optical modulation using Rb vapor confined to a hollow-core photonic bandgap fiber. The intensity of a signal field participating in the four-wave-mixing process is modulated using a weak switching field. We observe 3 dB of attenuation in the signal field with only 3600 photons of switching energy, corresponding to 23 photons per atomic cross section lambda(2)/(2pi).

Ultralow-power four-wave mixing with Rb in a hollow-core photonic band-gap fiber.

We demonstrate extremely efficient four-wave mixing with gains greater than 100 at microwatt pump powers and signal-to-idler conversion of 50% in Rb vapor confined to a hollow-core photonic band-gap fiber. We present a theoretical model that demonstrates such efficiency is consistent with the dimensions of the fiber and the optical depths attained. This is, to our knowledge, the largest four-wave mixing gain observed at such low total pump powers and the first demonstrated example of four-wave mixing in an alkali-metal vapor system with a large (approximately 30 MHz) ground state decoherence rate.

Generation of large alkali vapor densities inside bare hollow-core photonic band-gap fibers.

We demonstrate the ability to generate extremely large rubidium densities in uncoated hollow-core photonic band-gap fibers using light-induced atomic desorption. Once the fiber is exposed to Rb vapor for 1-2 weeks, and this atomic source is removed, the fiber yields large desorbable densities for an extended period of time. We show that optical depths greater than e(-1200) can be created within seconds.

Nonlinear optics in hollow-core photonic bandgap fibers.

Hollow-core photonic-bandgap fibers provide a new geometry for the realization and enhancement of many nonlinear optical effects. Such fibers offer novel guidance and dispersion properties that provide an advantage over conventional fibers for various applications. In this review we summarize the nonlinear optics experiments that have been performed using these hollow-core fibers.

Low-light-level optical interactions with rubidium vapor in a photonic band-gap fiber.

We show that rubidium vapor can be produced within the core of a photonic band-gap fiber yielding an optical depth in excess of 2,000. Our technique for producing the vapor is based on coating the inner walls of the fiber core with organosilane and using light-induced atomic desorption to release Rb atoms into the core. As an initial demonstration of the potential of this system for supporting ultralow-level nonlinear optical interactions, we perform electromagnetically induced transparency with control-field powers in the nanowatt regime, which represents more than a 1,000-fold reduction from the power required for bulk, focused geometries.