Immunomodulatory effects of astragalus polysaccharide on human peripheral blood mononuclear cells co-cultured with cervical cancer cell line.

Objective: To investigate immunomodulatory effects of Astragalus polysaccharides (APS) on the co-culture of peripheral blood mononuclear cells (PBMCs) with HeLa cervical cancer cell line.

Methods: To assess the proliferation of PBMCs, carboxyfluorescein succinimidyl ester (CFSE)-labeled PBMCs were co-cultured with HeLa cells and treated with different concentrations of APS. Supernatants of cell culture were collected for cytokines assay via enzyme-linked immunosorbent assay (ELISA).

Evaluation of Poly(I:C) and combination of CpG ODN plus Montanide ISA adjuvants to enhance the efficacy of outer membrane vesicles as an acellular vaccine against Brucella melitensis infection in mice.

Brucellosis is the most common zoonotic disease worldwide and still there is no vaccine for human use. The commercial animal vaccines also have major problems that limit their use. Therefore, there is a need for an effective Brucella vaccine which is multivalent and produces a good protective immunity with minimal disadvantages.

Comparison of the protective immunity elicited by a Brucella cocktail protein vaccine (rL7/L12+rTOmp31+rSOmp2b) in two different adjuvant formulations in BALB/c mice.

In the present study, protective efficacy conferred by a cocktail protein consisted of Brucella L7/L12 ribosomal, truncated outer membrane protein 31 (TOmp31) and SOmp2b recombinant proteins in CpG ODN 1826+ Montanide ISA 70VG or Poly (I:C) adjuvants was evaluated and compared in BALB/c mice. Immunization of mice with both vaccine regimens elicited strong specific IgG responses (higher IgG2a titers over IgG1 titers), provided T helper1 (Th1) oriented immune responses and conferred protection levels compatible to the live vaccines against Brucella challenge. Vaccination of BALB/c mice with the cocktail protein in CpG ODN 1826+ Montanide ISA 70 V G adjuvants induced higher levels of antibody, IFN-γ/IL-2 and conferred more protection levels against B.